PMID- 23872211
OWN - NLM
STAT- MEDLINE
DCOM- 20140519
LR  - 20130909
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 85
DP  - 2013 Nov
TI  - Dispersive liquid-liquid microextraction followed by high-performance liquid 
      chromatography-ultraviolet detection to determination of opium alkaloids in human 
      plasma.
PG  - 14-20
LID - S0731-7085(13)00290-2 [pii]
LID - 10.1016/j.jpba.2013.06.030 [doi]
AB  - A novel, simple, rapid and sensitive dispersive liquid-liquid microextraction 
      method based on the solidification of floating organic drop (DLLME-SFO) combined 
      with high-performance liquid chromatography-ultraviolet detection (HPLC-UV) was 
      used to determine opium alkaloids in human plasma. During the extraction 
      procedure, plasma protein was precipitated by using a mixture of zinc sulfate 
      solution and acetonitrile. Some effective parameters on extraction were studied 
      and optimized. Under the optimum conditions (extraction solvent: 30.0 mul 
      1-undecanol; disperser solvent: 470 mul acetone; pH: 9; salt addition: 1%(w/v) 
      NaCl and extraction time: 0.5 min), calibration curves are linear in the range of 
      1.5-1000 mugl(-1) and limit of detections (LODs) are in the range of 0.5-5 
      mugl(-1). The relative standard deviations (RSDs) for 100 mugl(-1) of morphine and 
      codeine, 10.0 mugl(-1) of papaverine and 20.0 mugl(-1) of noscapine in diluted 
      human plasma are in the range of 4.3-7.4% (n=5). Finally, the method was 
      successfully applied in the determination of opium alkaloids in the actual human 
      plasma samples. The relative recoveries of plasma samples spiked with alkaloids 
      are 88-110.5%. The obtained results show that DLLME-SFO combined with HPLC-UV is 
      a fast and simple method for the determination of opium alkaloids in human 
      plasma.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Ahmadi-Jouibari, Toraj
AU  - Ahmadi-Jouibari T
AD  - Internist, Iran West Center for Drug Abuse Studies, Kermanshah University of 
      Medical Sciences, Kermanshah, Iran.
FAU - Fattahi, Nazir
AU  - Fattahi N
FAU - Shamsipur, Mojtaba
AU  - Shamsipur M
FAU - Pirsaheb, Meghdad
AU  - Pirsaheb M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130704
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Opiate Alkaloids)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Liquid Phase Microextraction/*methods
MH  - Opiate Alkaloids/*blood
MH  - Spectrophotometry, Ultraviolet
MH  - Ultraviolet Rays
OTO - NOTNLM
OT  - Dispersive liquid-liquid microextraction
OT  - Human plasma
OT  - Opium alkaloids
OT  - Solidification of floating organic drop
EDAT- 2013/07/23 06:00
MHDA- 2014/05/20 06:00
CRDT- 2013/07/23 06:00
PHST- 2013/05/20 00:00 [received]
PHST- 2013/06/23 00:00 [revised]
PHST- 2013/06/24 00:00 [accepted]
PHST- 2013/07/23 06:00 [entrez]
PHST- 2013/07/23 06:00 [pubmed]
PHST- 2014/05/20 06:00 [medline]
AID - S0731-7085(13)00290-2 [pii]
AID - 10.1016/j.jpba.2013.06.030 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2013 Nov;85:14-20. doi: 10.1016/j.jpba.2013.06.030. Epub 
      2013 Jul 4.