PMID- 23872360
OWN - NLM
STAT- MEDLINE
DCOM- 20131213
LR  - 20220317
IS  - 1096-0007 (Electronic)
IS  - 0014-4835 (Linking)
VI  - 115
DP  - 2013 Oct
TI  - Plasma rich in growth factors (PRGF-Endoret) stimulates corneal wound healing and 
      reduces haze formation after PRK surgery.
PG  - 153-61
LID - S0014-4835(13)00197-8 [pii]
LID - 10.1016/j.exer.2013.07.007 [doi]
AB  - This study evaluated the efficacy of Plasma rich in growth factors (PRGF-Endoret) 
      on the corneal wound healing process after Photorefractive keratectomy (PRK). To 
      address this, blood from three healthy donors was collected, centrifuged and, the 
      whole plasma column (WP) and the plasma fraction with the highest platelet 
      concentration (F3) were collected. The effects of F3 and WP on the proliferation 
      and migration of human corneal epithelial cells (HCE) were analyzed. PRK was 
      performed on C57BL/6 mice. Animals were divided in three treatment groups: 
      Control, F3, and WP. Corneal wound healing and haze formation were evaluated 
      macroscopically. Eyes were collected at 1, 2, 3, and 7 days after surgery, and 
      were processed for histological studies. Immunofluorescence was used to assess 
      cellular proliferation, apoptosis and myofibroblast transformation in the mouse 
      cornea. Results showed a significant increased on proliferation and wound healing 
      after F3 and WP treatment when compared with control group. In vivo studies 
      showed significant reduction on haze formation in mice treated with both 
      PRGF-Endoret formulations (F3 and WP). Histological studies showed an increase of 
      epithelial cell proliferation in corneas of control group, promoting an 
      epithelial hyperplasia. The number of SMA-positive cells (corresponding to 
      myofibroblast differentiation) was significantly lower in the PRGF-Endoret group 
      than in the control group, correlating with the higher transparence results 
      observed macroscopically in both PRGF-Endoret groups. According to this, it can 
      be concluded that PRGF-Endoret accelerates corneal tissue regeneration after PRK, 
      reducing haze formation.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Anitua, E
AU  - Anitua E
AD  - Biotechnology Institute (BTI), Vitoria, Spain. Electronic address: 
      eduardoanitua@eduardoanitua.com.
FAU - Muruzabal, F
AU  - Muruzabal F
FAU - Alcalde, I
AU  - Alcalde I
FAU - Merayo-Lloves, J
AU  - Merayo-Lloves J
FAU - Orive, G
AU  - Orive G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130718
PL  - England
TA  - Exp Eye Res
JT  - Experimental eye research
JID - 0370707
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Ophthalmic Solutions)
SB  - IM
MH  - Animals
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Corneal Injuries
MH  - Corneal Opacity/etiology/*prevention & control
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epithelium, Corneal/cytology/metabolism
MH  - Fluorescent Antibody Technique, Indirect
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Intercellular Signaling Peptides and Proteins/*physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Ophthalmic Solutions
MH  - *Photorefractive Keratectomy
MH  - Platelet-Rich Plasma/*physiology
MH  - Postoperative Complications/*prevention & control
MH  - Wound Healing/*physiology
OTO - NOTNLM
OT  - Corneal epithelial cells
OT  - ECM
OT  - EGF
OT  - ELISA
OT  - Enzyme-linked immunosorbent assay
OT  - Epithelial growth factor
OT  - Extracellular matrix
OT  - FBS
OT  - FGF
OT  - Fetal bovine serum
OT  - Fibroblast growth factor
OT  - HCE
OT  - NGF
OT  - Nerve growth factor
OT  - PBS
OT  - PDGF
OT  - PRGF-Endoret
OT  - PRK
OT  - PRP
OT  - Phosphate buffered saline
OT  - Photorefractive keratectomy
OT  - Plasma Rich in Growth Factors
OT  - Platelet-derived growth factor
OT  - SMA
OT  - Smooth muscle actin
OT  - TGF
OT  - TUNEL assay
OT  - Terminal deoxyribonucleotidyl transferase-mediated dUTP-fluorescein Nick-End 
      labeling assay
OT  - Transforming growth factor-beta
OT  - cornea
OT  - myofibroblast
OT  - plasma rich in growth factors
OT  - platelet-rich plasma
OT  - wound healing
EDAT- 2013/07/23 06:00
MHDA- 2013/12/18 06:00
CRDT- 2013/07/23 06:00
PHST- 2013/01/28 00:00 [received]
PHST- 2013/06/19 00:00 [revised]
PHST- 2013/07/09 00:00 [accepted]
PHST- 2013/07/23 06:00 [entrez]
PHST- 2013/07/23 06:00 [pubmed]
PHST- 2013/12/18 06:00 [medline]
AID - S0014-4835(13)00197-8 [pii]
AID - 10.1016/j.exer.2013.07.007 [doi]
PST - ppublish
SO  - Exp Eye Res. 2013 Oct;115:153-61. doi: 10.1016/j.exer.2013.07.007. Epub 2013 Jul 
      18.