PMID- 23872956
OWN - NLM
STAT- MEDLINE
DCOM- 20140317
LR  - 20211203
IS  - 1420-9071 (Electronic)
IS  - 1420-682X (Linking)
VI  - 71
IP  - 4
DP  - 2014 Feb
TI  - The HIF-1 transcription complex is essential for translational control of myeloid 
      hematopoietic cell function by maintaining mTOR phosphorylation.
PG  - 699-710
LID - 10.1007/s00018-013-1421-2 [doi]
AB  - Mammalian myeloid cells are crucial effectors of host innate immune defense. 
      Normal and pathological responses of these cells require adaptation to signaling 
      stress through the hypoxia-inducible factor 1 (HIF-1) transcription complex. 
      Adapted cells activate the mammalian target of rapamycin (mTOR), via S2448 
      phosphorylation, which induces de novo translation of vital signaling proteins. 
      However, the molecular mechanisms underlying this signaling dogma remain unclear. 
      Here, we demonstrate for the first time that inactivation of HIF-1, by silencing 
      its inducible alpha subunit, significantly decreases mTOR S2448 phosphorylation 
      caused by ligand-dependent activation of human myeloid leukemia cells. This shows 
      that HIF-1 is essential for the activation of mTOR and serves at a crucial 
      juncture of myeloid cell function in both in vitro and in vivo systems.
FAU - Yasinska, Inna M
AU  - Yasinska IM
AD  - Medway School of Pharmacy, University of Kent, Anson Building, Central Avenue, 
      Chatham Maritime, Kent, ME4 4TB, UK.
FAU - Gibbs, Bernhard F
AU  - Gibbs BF
FAU - Lall, Gurprit S
AU  - Lall GS
FAU - Sumbayev, Vadim V
AU  - Sumbayev VV
LA  - eng
PT  - Journal Article
DEP - 20130720
PL  - Switzerland
TA  - Cell Mol Life Sci
JT  - Cellular and molecular life sciences : CMLS
JID - 9705402
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Tlr2 protein, mouse)
RN  - 0 (Toll-Like Receptor 2)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - AMP-Activated Protein Kinases/metabolism
MH  - Animals
MH  - Blood Cells/metabolism
MH  - Cell Line
MH  - Enzyme Activation
MH  - Humans
MH  - Hypoxia-Inducible Factor 1/genetics/*metabolism
MH  - Male
MH  - Mice
MH  - Myeloid Cells/*metabolism
MH  - Phosphorylation
MH  - RNA Interference
MH  - RNA, Small Interfering/genetics
MH  - TOR Serine-Threonine Kinases/*metabolism
MH  - Toll-Like Receptor 2/metabolism
EDAT- 2013/07/23 06:00
MHDA- 2014/03/19 06:00
CRDT- 2013/07/23 06:00
PHST- 2013/06/06 00:00 [received]
PHST- 2013/07/04 00:00 [accepted]
PHST- 2013/07/04 00:00 [revised]
PHST- 2013/07/23 06:00 [entrez]
PHST- 2013/07/23 06:00 [pubmed]
PHST- 2014/03/19 06:00 [medline]
AID - 10.1007/s00018-013-1421-2 [doi]
PST - ppublish
SO  - Cell Mol Life Sci. 2014 Feb;71(4):699-710. doi: 10.1007/s00018-013-1421-2. Epub 
      2013 Jul 20.