PMID- 23873725
OWN - NLM
STAT- MEDLINE
DCOM- 20140528
LR  - 20130916
IS  - 1097-4547 (Electronic)
IS  - 0360-4012 (Linking)
VI  - 91
IP  - 10
DP  - 2013 Oct
TI  - N-methyl-D-aspartate receptors strongly regulate postsynaptic activity levels 
      during optic nerve regeneration.
PG  - 1263-79
LID - 10.1002/jnr.23246 [doi]
AB  - During development, neuronal activity is used as a cue to guide synaptic 
      rearrangements to refine connections. Many studies, especially in the visual 
      system, have shown that the N-methyl-D-aspartate receptor (NMDAr) plays a key 
      role in mediating activity-dependent refinement through long-term potentiation 
      (LTP)-like processes. Adult goldfish can regenerate their optic nerve and utilize 
      neuronal activity to generate precise topography in their projection onto tectum. 
      Although the NMDAr has been implicated in this process, its precise role in 
      regeneration has not been extensively studied. In examining NMDAr function during 
      regeneration, we found salient differences compared with development. By using 
      field excitatory postsynaptic potential (fEPSP) recordings, the contribution of 
      the NMDAr at the primary optic synapse was measured. In contrast to development, 
      no increase in NMDAr function was detectable during synaptic refinement. Unlike 
      development, LTP could not be reliably elicited during regeneration. 
      Unexpectedly, we found that NMDAr exerted a major effect on regulating ongoing 
      tectal (postsynaptic) activity levels during regeneration. Blocking NMDAr 
      strongly suppressed spontaneous activity during regeneration but had no 
      significant effect in the normal projection. This difference could be attributed 
      to an occlusion effect of strong optic drive in the normal projection, which 
      dominated ongoing tectal activity. During regeneration, this optic drive is 
      largely absent. Optic nerve stimulation further indicated that the NMDAr had 
      little effect on the ability of optic fibers to evoke early postsynaptic impulse 
      activity but was important for late network activity. These results indicate 
      that, during regeneration, the NMDAr may play a critical role in the homeostatic 
      regulation of ongoing activity and network excitability.
CI  - Copyright (c) 2013 Wiley Periodicals, Inc.
FAU - Kolls, Brad J
AU  - Kolls BJ
AD  - Department of Developmental and Cell Biology, University of California Irvine, 
      Irvine, California; Brain Injury Translational Research Center, Division of 
      Neurology, Duke University Medical Center, Durham, North Carolina.
FAU - Meyer, Ronald L
AU  - Meyer RL
LA  - eng
PT  - Journal Article
DEP - 20130719
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
SB  - IM
MH  - Animals
MH  - Electrophysiological Phenomena
MH  - Excitatory Postsynaptic Potentials/*physiology
MH  - Goldfish/*physiology
MH  - Nerve Regeneration/*physiology
MH  - Optic Nerve/*physiology
MH  - Receptors, N-Methyl-D-Aspartate/*physiology
MH  - Synapses/physiology
OTO - NOTNLM
OT  - NMDA receptor
OT  - axonal remodeling
OT  - long-term potentiation
OT  - optic nerve
OT  - regeneration
EDAT- 2013/07/23 06:00
MHDA- 2014/05/29 06:00
CRDT- 2013/07/23 06:00
PHST- 2012/11/29 00:00 [received]
PHST- 2013/03/12 00:00 [revised]
PHST- 2013/03/18 00:00 [accepted]
PHST- 2013/07/23 06:00 [entrez]
PHST- 2013/07/23 06:00 [pubmed]
PHST- 2014/05/29 06:00 [medline]
AID - 10.1002/jnr.23246 [doi]
PST - ppublish
SO  - J Neurosci Res. 2013 Oct;91(10):1263-79. doi: 10.1002/jnr.23246. Epub 2013 Jul 
      19.