PMID- 23875878
OWN - NLM
STAT- MEDLINE
DCOM- 20140929
LR  - 20220317
IS  - 1753-0407 (Electronic)
IS  - 1753-0393 (Print)
IS  - 1753-0407 (Linking)
VI  - 6
IP  - 2
DP  - 2014 Mar
TI  - Oxidants, antioxidants and mitochondrial function in non-proliferative diabetic 
      retinopathy.
PG  - 167-75
LID - 10.1111/1753-0407.12076 [doi]
AB  - BACKGROUND: Diabetic retinopathy (DR) is a preventable cause of visual 
      disability. The aims of the present study were to investigate levels and behavior 
      oxidative stress markers and mitochondrial function in non-proliferative DR 
      (NPDR) and to establish the correlation between the severity of NPDR and markers 
      of oxidative stress and mitochondrial function. METHODS: In a transverse 
      analysis, type 2 diabetes mellitus (T2DM) patients with mild, moderate and severe 
      non-proliferative DR (NPDR) were evaluated for markers of oxidative stress (i.e. 
      products of lipid peroxidation (LPO) and nitric oxide (NO) catabolites) and 
      antioxidant activity (i.e. total antioxidant capacity (TAC), catalase, and 
      glutathione peroxidase (GPx) activity of erythrocytes). Mitochondrial function 
      was also determined as the fluidity of the submitochondrial particles of 
      platelets and the hydrolytic activity of F0 /F1 -ATPase. RESULTS: Levels of LPO 
      and NO were significantly increased in T2DM patients with severe NPDR 
      (3.19 +/- 0.05 mumol/mL and 45.62 +/- 1.27 pmol/mL, respectively; P < 0.007 and 
      P < 0.0001 vs levels in health volunteers, respectively), suggesting the presence 
      of oxidative stress. TAC had significant decrease levels with minimum peak in 
      severe retinopathy with 7.98 +/- 0.48 mEq/mL (P < 0.0001). In contrast with TAC, 
      erythrocyte catalase and GPx activity was increased in patients with severe NPDR 
      (139.4 +/- 4.4 and 117.13 +/- 14.84 U/mg, respectively; P < 0.0001 vs healthy 
      volunteers for both), suggesting an imbalance between oxidants and antioxidants. 
      The fluidity of membrane submitochondrial particles decreased significantly in 
      T2DM patients with mild, moderate, or severe NPDR compared with that in healthy 
      volunteers (P < 0.0001 for all). Furthermore, there was a significant increase in 
      the hydrolytic activity of the F0 /F1 -ATPase in T2DM patients with mild NPDR 
      (265.07 +/- 29.55 nmol/PO4 ; P < 0.0001 vs healthy volunteers), suggesting 
      increased catabolism. CONCLUSIONS: Patients with NPDR exhibit oxidative 
      deregulation with decreased membrane fluidity of submitochondrial particles and 
      increased systemic catabolism (mitochondrial dysfunction) with the potential for 
      generalized systemic damage in T2DM.
CI  - (c) 2013 The Authors. Journal of Diabetes published by Ruijin Hospital, Shanghai 
      Jiaotong University School of Medicine and Wiley Publishing Asia Pty Ltd.
FAU - Rodriguez-Carrizalez, Adolfo Daniel
AU  - Rodriguez-Carrizalez AD
AD  - University Health Sciences Centre, University of Guadalajara, Guadalajara, 
      Mexico.
FAU - Castellanos-Gonzalez, Jose Alberto
AU  - Castellanos-Gonzalez JA
FAU - Martinez-Romero, Esau Cesar
AU  - Martinez-Romero EC
FAU - Miller-Arrevillaga, Guillermo
AU  - Miller-Arrevillaga G
FAU - Villa-Hernandez, David
AU  - Villa-Hernandez D
FAU - Hernandez-Godinez, Pedro Pablo
AU  - Hernandez-Godinez PP
FAU - Ortiz, Genaro Gabriel
AU  - Ortiz GG
FAU - Pacheco-Moises, Fermin Paul
AU  - Pacheco-Moises FP
FAU - Cardona-Munoz, Ernesto German
AU  - Cardona-Munoz EG
FAU - Miranda-Diaz, Alejandra Guillermina
AU  - Miranda-Diaz AG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130821
PL  - Australia
TA  - J Diabetes
JT  - Journal of diabetes
JID - 101504326
RN  - 0 (Antioxidants)
RN  - 0 (Oxidants)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 3.6.3.14 (Proton-Translocating ATPases)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Adult
MH  - Aged
MH  - Antioxidants/*metabolism
MH  - Catalase/metabolism
MH  - Diabetes Mellitus, Type 2/blood/complications/*metabolism
MH  - Diabetic Retinopathy/blood/etiology/*metabolism
MH  - Erythrocytes/metabolism
MH  - Female
MH  - Glutathione Peroxidase/metabolism
MH  - Humans
MH  - Hydrolysis
MH  - Lipid Peroxidation
MH  - Male
MH  - Membrane Fluidity
MH  - Middle Aged
MH  - Mitochondria/metabolism/*physiology
MH  - Mitochondrial Membranes/chemistry/metabolism
MH  - Multivariate Analysis
MH  - Nitric Oxide/metabolism
MH  - Oxidants/*metabolism
MH  - Proton-Translocating ATPases/metabolism
PMC - PMC4232896
OTO - NOTNLM
OT  - diabetes mellitus
OT  - diabetic retinopathy
OT  - membrane fluidity
OT  - nitrosative stress
OT  - oxidative stress
OT  - 糖尿病，糖尿病视网膜病变，膜流动性，硝化应激，氧化应激
EDAT- 2013/07/24 06:00
MHDA- 2014/09/30 06:00
PMCR- 2014/11/15
CRDT- 2013/07/24 06:00
PHST- 2013/05/29 00:00 [received]
PHST- 2013/07/14 00:00 [accepted]
PHST- 2013/07/24 06:00 [entrez]
PHST- 2013/07/24 06:00 [pubmed]
PHST- 2014/09/30 06:00 [medline]
PHST- 2014/11/15 00:00 [pmc-release]
AID - 10.1111/1753-0407.12076 [doi]
PST - ppublish
SO  - J Diabetes. 2014 Mar;6(2):167-75. doi: 10.1111/1753-0407.12076. Epub 2013 Aug 21.