PMID- 23876303
OWN - NLM
STAT- MEDLINE
DCOM- 20140613
LR  - 20171116
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 155
IP  - 3
DP  - 2013 Sep 15
TI  - The stimulatory effect of TLRs ligands on maturation of chicken bone 
      marrow-derived dendritic cells.
PG  - 205-10
LID - S0165-2427(13)00196-7 [pii]
LID - 10.1016/j.vetimm.2013.06.014 [doi]
AB  - Dendritic cells (DCs) are crucial for initiation of both innate and adaptive 
      immune responses. TLR ligands combine with Toll-like receptors (TLRs) expressed 
      on the DC surface and induce DC maturation. The potential effect of three types 
      of TLR ligands (Bacillus subtilis (B. subtilis) spores, 
      polyinosinic-polycytidylic acid and CpG oligodeoxynucleotides) on chicken bone 
      marrow-derived DCs (chBM-DCs) maturation was studied. The chBM-DCs cultured in 
      presence of recombinant chicken granulocyte-macrophage colony-stimulating factor 
      (GM-CSF) and interleukin (IL)-4 displayed the typical morphology of DCs after 7 
      days of culture. These immature chBM-DCs up-regulated the expression of MHC-II 
      and of the putative CD11c, but had yet low to moderate levels of the CD40 and 
      CD86 co-stimulatory molecules. After stimulation by the TLR ligands, the chBM-DCs 
      displayed a more mature morphologic phenotype, significantly increased the CD40 
      and CD86 cell surface expression levels and gained the ability to stimulate 
      proliferation of naive T cells in the allogeneic mixed lymphocyte reaction, 
      compared to the immature chBM-DCs. In conclusion, our data demonstrated that all 
      three TLR ligands were strong stimuli for driving chBM-DCs maturation in vitro, 
      with B. subtilis spores being the most efficient.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Liang, Jinfeng
AU  - Liang J
AD  - Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing 
      Agricultural University, Weigang 1, Nanjing, Jiangsu 210095, China.
FAU - Fu, Jia
AU  - Fu J
FAU - Kang, Haihong
AU  - Kang H
FAU - Lin, Jian
AU  - Lin J
FAU - Yu, Qinghua
AU  - Yu Q
FAU - Yang, Qian
AU  - Yang Q
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130628
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (CPG-oligonucleotide)
RN  - 0 (Ligands)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Toll-Like Receptors)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - O84C90HH2L (Poly I-C)
SB  - IM
MH  - Animals
MH  - Bacillus subtilis/physiology
MH  - Bone Marrow Cells/*physiology
MH  - Chickens/*immunology
MH  - Dendritic Cells/*physiology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Interleukin-4/pharmacology
MH  - Ligands
MH  - Lymphocyte Activation
MH  - Oligodeoxyribonucleotides/pharmacology
MH  - Poly I-C/pharmacology
MH  - Spleen/cytology
MH  - Spores, Bacterial/physiology
MH  - Toll-Like Receptors/*physiology
OTO - NOTNLM
OT  - Bone marrow
OT  - Chicken
OT  - Dendritic cells
OT  - TLR ligands
EDAT- 2013/07/24 06:00
MHDA- 2014/06/15 06:00
CRDT- 2013/07/24 06:00
PHST- 2013/03/09 00:00 [received]
PHST- 2013/06/10 00:00 [revised]
PHST- 2013/06/24 00:00 [accepted]
PHST- 2013/07/24 06:00 [entrez]
PHST- 2013/07/24 06:00 [pubmed]
PHST- 2014/06/15 06:00 [medline]
AID - S0165-2427(13)00196-7 [pii]
AID - 10.1016/j.vetimm.2013.06.014 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2013 Sep 15;155(3):205-10. doi: 
      10.1016/j.vetimm.2013.06.014. Epub 2013 Jun 28.