PMID- 23876312
OWN - NLM
STAT- MEDLINE
DCOM- 20131104
LR  - 20161125
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 438
IP  - 1
DP  - 2013 Aug 16
TI  - Oxysterols induce transition of monocytic cells to phenotypically mature 
      dendritic cell-like cells.
PG  - 161-8
LID - S0006-291X(13)01197-2 [pii]
LID - 10.1016/j.bbrc.2013.07.046 [doi]
AB  - Dendritic cells (DCs) activate adaptive immune responses in atherosclerotic 
      plaques; however, the origin of DCs is in question. We attempted to determine 
      whether cholesterol or its oxide forms, which are detected in abundance in 
      atheromatous lesions, could induce differentiation or transition of monocytic 
      cells to DCs. Treatment of THP-1 cells with 27-hydroxycholesterol (27OH-Chol) and 
      7alpha-hydroxycholesterol (7alphaOH-Chol) resulted in an increase in the numbers of 
      adherent cells, and, in contrast to PMA, decreased uptake of FITC-conjugated 
      dextran. In addition, treatment with 27OH-Chol and 7alphaOH-Chol induced expression 
      of mDC-specific molecules, including CD40, CD80, CD83, and CD88. Of the two 
      oxysterols, 27OH-Chol enhanced expression of MHC class I and II molecules as well 
      as CCR7. However, treatment with an identical concentration of cholesterol and 
      7-ketocholesterol did not influence adherence, uptake of FITC-conjugated dextran, 
      and expression of the aforementioned molecules. This is the first study to report 
      on change of monocytic cells by oxysterols to phenotypically atypical cells with 
      some characteristics of mDCs detected in atherosclerotic lesions. We propose that 
      a certain type of oxysterol would contribute to immune responses in 
      atherosclerotic lesions by enhancing expression of multiple CD molecules as well 
      as MHC molecules by monocytic cells.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Son, Yonghae
AU  - Son Y
AD  - Department of Pharmacology, School of Medicine, Pusan National University, 
      Yangsan 626-870, Republic of Korea.
FAU - Kim, Sun-Mi
AU  - Kim SM
FAU - Lee, Sae-A
AU  - Lee SA
FAU - Eo, Seong-Kug
AU  - Eo SK
FAU - Kim, Koanhoi
AU  - Kim K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130720
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Hydroxycholesterols)
RN  - 566-26-7 (cholest-5-en-3 beta,7 alpha-diol)
RN  - 6T2NA6P5SQ (27-hydroxycholesterol)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/*drug effects/immunology
MH  - Cell Line
MH  - Dendritic Cells/*cytology/*drug effects/immunology
MH  - Humans
MH  - Hydroxycholesterols/*pharmacology
MH  - Mice
MH  - Monocytes/*cytology/*drug effects/immunology
MH  - Phenotype
OTO - NOTNLM
OT  - Atherosclerosis
OT  - Dendritic cells
OT  - Monocytes
OT  - Oxysterols
EDAT- 2013/07/24 06:00
MHDA- 2013/11/05 06:00
CRDT- 2013/07/24 06:00
PHST- 2013/07/11 00:00 [received]
PHST- 2013/07/12 00:00 [accepted]
PHST- 2013/07/24 06:00 [entrez]
PHST- 2013/07/24 06:00 [pubmed]
PHST- 2013/11/05 06:00 [medline]
AID - S0006-291X(13)01197-2 [pii]
AID - 10.1016/j.bbrc.2013.07.046 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2013 Aug 16;438(1):161-8. doi: 
      10.1016/j.bbrc.2013.07.046. Epub 2013 Jul 20.