PMID- 23876802
OWN - NLM
STAT- MEDLINE
DCOM- 20131125
LR  - 20211021
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Print)
IS  - 0019-9567 (Linking)
VI  - 81
IP  - 10
DP  - 2013 Oct
TI  - DC-LAMP+ dendritic cells are recruited to gastric lymphoid follicles in 
      Helicobacter pylori-infected individuals.
PG  - 3684-92
LID - 10.1128/IAI.00801-13 [doi]
AB  - Infection with Helicobacter pylori is associated with development of ulcer 
      disease and gastrointestinal adenocarcinoma. The infection leads to a large 
      infiltration of immune cells and the formation of organized lymphoid follicles in 
      the human gastric mucosa. Still, the immune system fails to eradicate the 
      bacteria, and the substantial regulatory T cell (Treg) response elicited is 
      probably a major factor permitting bacterial persistence. Dendritic cells (DCs) 
      are professional antigen-presenting cells that can activate naive T cells, and 
      maturation of DCs is crucial for the initiation of primary immune responses. The 
      aim of this study was to investigate the presence and localization of mature 
      human DCs in H. pylori-infected gastric mucosa. Gastric antral biopsy specimens 
      were collected from patients with H. pylori-associated gastritis and healthy 
      volunteers, and antrum tissue was collected from patients undergoing gastric 
      resection. Immunohistochemistry and flow cytometry showed that DCs expressing the 
      maturation marker dendritic cell lysosome-associated membrane glycoprotein 
      (DC-LAMP; CD208) are enriched in the H. pylori-infected gastric mucosa and that 
      these DCs are specifically localized within or close to lymphoid follicles. 
      Gastric DC-LAMP-positive (DC-LAMP(+)) DCs express CD11c and high levels of HLA-DR 
      but little CD80, CD83, and CD86. Furthermore, immunofluorescence analyses 
      demonstrated that DC-LAMP(+) DCs are in the same location as FoxP3-positive 
      putative Tregs in the follicles. In conclusion, we show that DC-LAMP(+) DCs with 
      low costimulatory capacity accumulate in the lymphoid follicles in human H. 
      pylori-infected gastric tissue, and our results suggest that Treg-DC interactions 
      may promote chronic infection by rendering gastric DCs tolerogenic.
FAU - Hansson, Malin
AU  - Hansson M
AD  - Department of Microbiology and Immunology, Institute of Biomedicine, University 
      of Gothenburg, and Goteborg University Vaccine Research Institute (GUVAX) and the 
      Mucosal Immunobiology and Vaccine Center (MIVAC), Goteborg, Sweden.
FAU - Sundquist, Malin
AU  - Sundquist M
FAU - Hering, Susanne
AU  - Hering S
FAU - Lundin, B Samuel
AU  - Lundin BS
FAU - Hermansson, Michael
AU  - Hermansson M
FAU - Quiding-Jarbrink, Marianne
AU  - Quiding-Jarbrink M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130722
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Lysosomal-Associated Membrane Protein 3)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Case-Control Studies
MH  - Dendritic Cells/*metabolism/physiology
MH  - Female
MH  - Gastritis/microbiology/pathology
MH  - Gene Expression Regulation/*immunology
MH  - Helicobacter Infections/*microbiology
MH  - *Helicobacter pylori
MH  - Humans
MH  - Inflammation/metabolism/microbiology
MH  - Lysosomal-Associated Membrane Protein 3/genetics/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Stomach
MH  - Young Adult
PMC - PMC3811775
EDAT- 2013/07/24 06:00
MHDA- 2013/12/16 06:00
PMCR- 2014/04/01
CRDT- 2013/07/24 06:00
PHST- 2013/07/24 06:00 [entrez]
PHST- 2013/07/24 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
PHST- 2014/04/01 00:00 [pmc-release]
AID - IAI.00801-13 [pii]
AID - 00801-13 [pii]
AID - 10.1128/IAI.00801-13 [doi]
PST - ppublish
SO  - Infect Immun. 2013 Oct;81(10):3684-92. doi: 10.1128/IAI.00801-13. Epub 2013 Jul 
      22.