PMID- 23877278
OWN - NLM
STAT- MEDLINE
DCOM- 20140327
LR  - 20211203
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 32
IP  - 4
DP  - 2013 Oct
TI  - Ghrelin attenuates intestinal ischemia/reperfusion injury in mice by activating 
      the mTOR signaling pathway.
PG  - 851-9
LID - 10.3892/ijmm.2013.1452 [doi]
AB  - Intestinal ischemia/reperfusion (I/R) injury is a serious condition in intensive 
      care patients, resulting in severe inflammation and remote organ damage. The 
      activation of the mammalian target of rapamycin (mTOR)/p70 ribosomal S6 kinase 
      (p70S6K) signaling pathway exerts protective effect against ischemia/reperfusion 
      injury. Ghrelin, an orexigenic hormone, inhibits the release of pro-inflammatory 
      cytokines, such as interleukin (IL)-1beta, tumor necrosis factor-alpha and IL-6. In this 
      study, we investigated the effects of ghrelin on gut I/R injury and the 
      regulation of the mTOR/p70S6K signaling pathway following gut I/R injury in mice. 
      C57BL/6 mice underwent superior mesenteric artery occlusion for 45 min, followed 
      by reperfusion for 4 h. Ghrelin was administered at the onset of reperfusion. We 
      assessed survival, organ injury variables, pro-inflammatory cytokine expression 
      and observed the histological changes of the small intestine and lungs. Our 
      results revealed that the administration of ghrelin inhibited the release of 
      certain pro-inflammatory cytokines, reduced neutrophil infiltration, attenuated 
      organ injury and improved survival following gut I/R injury. The administration 
      of D-Lys-GHRP6, a specific ghrelin receptor antagonist, to a certain extent, 
      counteracted the protective effects of ghrelin in gut I/R-induced organ injury 
      and mortality. To determine whether the beneficial effects of ghrelin following 
      gut I/R injury are associated with the mTOR/p70S6K signaling pathway, the 
      phosphorylation levels of mTOR and p70S6K were detected by western blot analysis. 
      Our results revealed that mTOR and p70S6K phosphorylation increased in the tissue 
      from the small intestine and pulmonary tissue in the animals treated with 
      ghrelin. These findings suggest that ghrelin attenuates organ injury following 
      gut I/R by promoting the activation of the mTOR/p70S6K signaling pathway.
FAU - Zhang, Hongwei
AU  - Zhang H
AD  - Laboratory of Clinical Immunology, Wuhan No. 1 Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, Hubei 430022, PR China.
FAU - Cui, Zhongyi
AU  - Cui Z
FAU - Luo, Guangwei
AU  - Luo G
FAU - Zhang, Jiaheng
AU  - Zhang J
FAU - Ma, Tao
AU  - Ma T
FAU - Hu, Na
AU  - Hu N
FAU - Cui, Tianpen
AU  - Cui T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130719
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Ghrelin)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - EC 2.7.1.1 (mTOR protein, mouse)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Ghrelin/blood/*pharmacology
MH  - Inflammation/drug therapy/pathology
MH  - Interleukin-1beta/blood
MH  - Interleukin-6/blood
MH  - Intestines/*drug effects/pathology
MH  - Lung/drug effects/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neutrophil Infiltration/drug effects
MH  - Peroxidase/metabolism
MH  - Phosphorylation
MH  - Reperfusion Injury/*drug therapy/pathology
MH  - Ribosomal Protein S6 Kinases, 70-kDa/genetics/metabolism
MH  - *Signal Transduction
MH  - TOR Serine-Threonine Kinases/*genetics/metabolism
MH  - Tumor Necrosis Factor-alpha/blood
EDAT- 2013/07/24 06:00
MHDA- 2014/03/29 06:00
CRDT- 2013/07/24 06:00
PHST- 2013/02/28 00:00 [received]
PHST- 2013/05/15 00:00 [accepted]
PHST- 2013/07/24 06:00 [entrez]
PHST- 2013/07/24 06:00 [pubmed]
PHST- 2014/03/29 06:00 [medline]
AID - 10.3892/ijmm.2013.1452 [doi]
PST - ppublish
SO  - Int J Mol Med. 2013 Oct;32(4):851-9. doi: 10.3892/ijmm.2013.1452. Epub 2013 Jul 
      19.