PMID- 23877360
OWN - NLM
STAT- MEDLINE
DCOM- 20140429
LR  - 20220408
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 30
IP  - 4
DP  - 2013 Oct
TI  - Apoptosis induced by PGC-1beta in breast cancer cells is mediated by the mTOR 
      pathway.
PG  - 1631-8
LID - 10.3892/or.2013.2628 [doi]
AB  - The peroxisome proliferator-activated receptor-gamma (PPAR-gamma) coactivator-1beta (PGC-1beta) 
      is a well-established regulator of mitochondrial biogenesis. However, the 
      underlying mechanism of PGC-1beta action remains elusive. This study reveals that 
      knockdown of endogenous PGC-1beta by short-hairpin RNA (shRNA) leads to a decrease 
      in the expression of mammalian target of rapamycin (mTOR) pathway-related genes 
      in MDA-MB-231 cells. After knockdown of PGC-1beta, phosphorylation of AMP-activated 
      protein kinase (AMPK), phosphorylation of Rictor on Thr1135, Raptor and S6 
      protein was inhibited. However, Akt phosphorylation on Ser473 was upregulated and 
      cell apoptosis occurred. In particular, we demonstrate that the levels of PGC-1beta 
      and mTOR correlated with overall mitochondrial activity. These results provide 
      new evidence that cell apoptosis is orchestrated by the balance between several 
      signaling pathways, and that PGC-1beta takes part in these events in breast cancer 
      cells mediated by the mTOR signaling pathway.
FAU - Wang, Libin
AU  - Wang L
AD  - Life Science College, Shaanxi Normal University, Xi'an 710062, P.R. China.
FAU - Liu, Qilun
AU  - Liu Q
FAU - Li, Fang
AU  - Li F
FAU - Qiu, Jing
AU  - Qiu J
FAU - Fan, Heng
AU  - Fan H
FAU - Ma, Haibin
AU  - Ma H
FAU - Zhu, Yongzhao
AU  - Zhu Y
FAU - Wu, Ligang
AU  - Wu L
FAU - Han, Xuebo
AU  - Han X
FAU - Yang, Zhihong
AU  - Yang Z
FAU - Jiang, Haifeng
AU  - Jiang H
FAU - Wei, Jun
AU  - Wei J
FAU - Xia, Haibin
AU  - Xia H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130718
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (BECN1 protein, human)
RN  - 0 (Beclin-1)
RN  - 0 (Carrier Proteins)
RN  - 0 (MAP1LC3A protein, human)
RN  - 0 (Membrane Proteins)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Multiprotein Complexes)
RN  - 0 (PPARGC1B protein, human)
RN  - 0 (RICTOR protein, human)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (RPTOR protein, human)
RN  - 0 (Rapamycin-Insensitive Companion of mTOR Protein)
RN  - 0 (Regulatory-Associated Protein of mTOR)
RN  - 415SHH325A (Adenosine Monophosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (Mechanistic Target of Rapamycin Complex 1)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - AMP-Activated Protein Kinases/metabolism
MH  - Adaptor Proteins, Signal Transducing/metabolism
MH  - Adenosine Monophosphate/metabolism
MH  - Adenosine Triphosphate/metabolism
MH  - Apoptosis/*genetics
MH  - Apoptosis Regulatory Proteins/biosynthesis
MH  - Beclin-1
MH  - Breast Neoplasms/*metabolism
MH  - Carrier Proteins/genetics/*metabolism
MH  - Female
MH  - HEK293 Cells
MH  - Humans
MH  - Mechanistic Target of Rapamycin Complex 1
MH  - Membrane Proteins/biosynthesis
MH  - Microtubule-Associated Proteins/metabolism
MH  - Mitochondria/metabolism
MH  - Multiprotein Complexes/metabolism
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - RNA Interference
MH  - RNA, Small Interfering
MH  - RNA-Binding Proteins
MH  - Rapamycin-Insensitive Companion of mTOR Protein
MH  - Regulatory-Associated Protein of mTOR
MH  - Ribosomal Protein S6 Kinases/metabolism
MH  - Signal Transduction/genetics
MH  - TOR Serine-Threonine Kinases/biosynthesis/*metabolism
EDAT- 2013/07/24 06:00
MHDA- 2014/04/30 06:00
CRDT- 2013/07/24 06:00
PHST- 2013/02/17 00:00 [received]
PHST- 2013/04/19 00:00 [accepted]
PHST- 2013/07/24 06:00 [entrez]
PHST- 2013/07/24 06:00 [pubmed]
PHST- 2014/04/30 06:00 [medline]
AID - 10.3892/or.2013.2628 [doi]
PST - ppublish
SO  - Oncol Rep. 2013 Oct;30(4):1631-8. doi: 10.3892/or.2013.2628. Epub 2013 Jul 18.