PMID- 23879967
OWN - NLM
STAT- MEDLINE
DCOM- 20131030
LR  - 20171116
IS  - 1872-7980 (Electronic)
IS  - 0304-3835 (Linking)
VI  - 339
IP  - 1
DP  - 2013 Oct 1
TI  - A novel interaction between calcium-modulating cyclophilin ligand and Basigin 
      regulates calcium signaling and matrix metalloproteinase activities in human 
      melanoma cells.
PG  - 93-101
LID - S0304-3835(13)00539-9 [pii]
LID - 10.1016/j.canlet.2013.07.019 [doi]
AB  - Intracellular free calcium is a ubiquitous second messenger regulating a 
      multitude of normal and pathogenic cellular responses, including the development 
      of melanoma. Upstream signaling pathways regulating the intracellular free 
      calcium concentration ([Ca2+]i) may therefore have a significant impact on 
      melanoma growth and metastasis. In this study, we demonstrate that the 
      endoplasmic reticulum (ER)-associated protein calcium-modulating cyclophilin 
      ligand (CAML) is bound to Basigin, a widely expressed integral plasma membrane 
      glycoprotein and extracellular matrix metalloproteinase inducer (EMMPRIN, or 
      CD147) implicated in melanoma proliferation, invasiveness, and metastasis. This 
      interaction between CAML and Basigin was first identified using yeast two-hybrid 
      screening and further confirmed by co-immunoprecipitation. In human A375 melanoma 
      cells, CAML and Basigin were co-localized to the ER. Knockdown of Basigin in 
      melanoma cells by siRNA significantly decreased resting [Ca2+]i and the [Ca2+]i 
      increase induced by the sarco/endoplasmic reticulum Ca(2+)-ATPase (SERCA) 
      inhibitor thapsigargin (TG), indicating that the interaction between CAML and 
      Basigin regulates ER-dependent [Ca2+]i signaling. Meanwhile upregulating the 
      [Ca2+]i either by TG or phorbol myristate acetate (PMA) could stimulate the 
      production of MMP-9 in A375 cells with the expression of Basigin. Our study has 
      revealed a previously uncharacterized [Ca2+]i signaling pathway that may control 
      melanoma invasion, and metastasis. Disruption of this pathway may be a novel 
      therapeutic strategy for melanoma treatment.
CI  - Copyright (c) 2013 Elsevier Ireland Ltd. All rights reserved.
FAU - Long, Tingting
AU  - Long T
AD  - Department of Dermatology, XiangYa Hospital, Central South University, Changsha, 
      Hunan, China.
FAU - Su, Juan
AU  - Su J
FAU - Tang, Wen
AU  - Tang W
FAU - Luo, Zhongling
AU  - Luo Z
FAU - Liu, Shuang
AU  - Liu S
FAU - Liu, Zhaoqian
AU  - Liu Z
FAU - Zhou, Honghao
AU  - Zhou H
FAU - Qi, Min
AU  - Qi M
FAU - Zeng, Weiqi
AU  - Zeng W
FAU - Zhang, Jianglin
AU  - Zhang J
FAU - Chen, Xiang
AU  - Chen X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130720
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (CAMLG protein, human)
RN  - 136894-56-9 (Basigin)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/chemistry/*metabolism
MH  - Basigin/chemistry/genetics/*metabolism
MH  - Calcium/metabolism
MH  - *Calcium Signaling
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Intracellular Space/metabolism
MH  - Matrix Metalloproteinase 9/genetics/metabolism
MH  - Matrix Metalloproteinases/*metabolism
MH  - Melanoma/genetics/*metabolism
MH  - Protein Binding
MH  - Protein Interaction Domains and Motifs
MH  - Protein Transport
OTO - NOTNLM
OT  - Basigin
OT  - CAML
OT  - Calcium
OT  - D105-199
OT  - D207-230
OT  - D207-269
OT  - D231-269
OT  - D34-89
OT  - EMMPRIN
OT  - FL
OT  - IgG
OT  - MMP
OT  - Melanoma
OT  - NF-AT
OT  - OPNG
OT  - PMA
OT  - SERCA
OT  - TACI
OT  - TG
OT  - WCL
OT  - calcium modulating cyclophilin ligand
OT  - co-IP
OT  - co-immunoprecipitation
OT  - deletion of the cytoplasmic domain
OT  - deletion of the first IgG
OT  - deletion of the second IgG
OT  - deletion of the transmembrane and cytoplasmic domain
OT  - deletion of the transmembrane domain
OT  - extracellular matrix metalloproteinase inducer
OT  - full length
OT  - immunoglobulin
OT  - mIgG
OT  - matrix metalloproteinase
OT  - mouse IgG
OT  - ortho-nitrophenyl-beta-D-galactopyranoside
OT  - phorbol myristate acetate
OT  - sarco/endoplasmic reticulum Ca(2+)-ATPase
OT  - si-RNA
OT  - small interfering RNA
OT  - thapsigargin
OT  - the nuclear factor of activated T cells transcription factor
OT  - transmembrane activator and CAML-interactor
OT  - whole cell lysate
EDAT- 2013/07/25 06:00
MHDA- 2013/10/31 06:00
CRDT- 2013/07/25 06:00
PHST- 2013/05/08 00:00 [received]
PHST- 2013/07/11 00:00 [revised]
PHST- 2013/07/15 00:00 [accepted]
PHST- 2013/07/25 06:00 [entrez]
PHST- 2013/07/25 06:00 [pubmed]
PHST- 2013/10/31 06:00 [medline]
AID - S0304-3835(13)00539-9 [pii]
AID - 10.1016/j.canlet.2013.07.019 [doi]
PST - ppublish
SO  - Cancer Lett. 2013 Oct 1;339(1):93-101. doi: 10.1016/j.canlet.2013.07.019. Epub 
      2013 Jul 20.