PMID- 23880219
OWN - NLM
STAT- MEDLINE
DCOM- 20140616
LR  - 20221207
IS  - 1879-2448 (Electronic)
IS  - 0043-1354 (Linking)
VI  - 47
IP  - 18
DP  - 2013 Nov 15
TI  - Bacteroidales terminal restriction fragment length polymorphism (TRFLP) for fecal 
      source differentiation in comparison to and in combination with universal 
      bacteria TRFLP.
PG  - 6944-55
LID - S0043-1354(13)00543-5 [pii]
LID - 10.1016/j.watres.2013.03.060 [doi]
AB  - Terminal restriction fragment length polymorphism (TRFLP) is an attractive 
      community analysis method for microbial source tracking (MST) because it is 
      accessible, relatively inexpensive, and can discern multiple fecal sources 
      simultaneously. A new Bacteroidales TRFLP (Bac-TRFLP) method was developed and 
      its source identification performance was evaluated by itself, in comparison to, 
      and in combination with an existing universal bacterial TRFLP method in two 
      laboratories. Sixty-four blind samples from 12 fecal sources (sewage, septage, 
      human, dog, horse, cow, deer, pig, chicken, goose, pigeon, and gull) were used 
      for evaluation. Bac- and Univ-TRFLP exhibited similarly high overall correct 
      identification (>88% and >89%, respectively), excellent specificity regardless of 
      fecal sources, variable sensitivity depending on the source, and stable 
      performance across two laboratories. Compared to Univ-TRFLP, Bac-TRFLP had better 
      sensitivity and specificity with horse, cow, and pig fecal sources but was not 
      suited for certain avian sources such as goose, gull, and pigeon. Combining the 
      general and more targeted TRFLP methods (Univ&Bac-TRFLP) achieved higher overall 
      correct identification (>92%), higher sensitivity and specificity metrics, and 
      higher reproducibility between laboratories. Our results suggest that the 
      Bac-TRFLP and Univ&Bac-TRFLP methods are promising additions to the MST toolbox 
      and warrant further evaluation and utilization in field MST applications.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Cao, Yiping
AU  - Cao Y
AD  - Southern California Coastal Water Research Project Authority, Costa Mesa, CA 
      92626, USA.
FAU - Van De Werfhorst, Laurie C
AU  - Van De Werfhorst LC
FAU - Scott, Elizabeth A
AU  - Scott EA
FAU - Raith, Meredith R
AU  - Raith MR
FAU - Holden, Patricia A
AU  - Holden PA
FAU - Griffith, John F
AU  - Griffith JF
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130704
PL  - England
TA  - Water Res
JT  - Water research
JID - 0105072
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Waste Water)
SB  - IM
MH  - Animals
MH  - Bacteroidetes/*classification/*genetics/isolation & purification/metabolism
MH  - Birds/microbiology
MH  - DNA, Bacterial/classification/genetics/metabolism
MH  - Environmental Monitoring/*methods
MH  - Feces/microbiology
MH  - Humans
MH  - Mammals/microbiology
MH  - Polymerase Chain Reaction/*methods
MH  - *Polymorphism, Restriction Fragment Length
MH  - RNA, Ribosomal, 16S/classification/genetics/metabolism
MH  - Sensitivity and Specificity
MH  - Wastewater/*microbiology
OTO - NOTNLM
OT  - Bacteroidales
OT  - Community analysis
OT  - Fecal source identification
OT  - TRFLP
EDAT- 2013/07/25 06:00
MHDA- 2014/06/17 06:00
CRDT- 2013/07/25 06:00
PHST- 2012/10/08 00:00 [received]
PHST- 2013/03/04 00:00 [revised]
PHST- 2013/03/17 00:00 [accepted]
PHST- 2013/07/25 06:00 [entrez]
PHST- 2013/07/25 06:00 [pubmed]
PHST- 2014/06/17 06:00 [medline]
AID - S0043-1354(13)00543-5 [pii]
AID - 10.1016/j.watres.2013.03.060 [doi]
PST - ppublish
SO  - Water Res. 2013 Nov 15;47(18):6944-55. doi: 10.1016/j.watres.2013.03.060. Epub 
      2013 Jul 4.