PMID- 23882691
OWN - NLM
STAT- MEDLINE
DCOM- 20131031
LR  - 20211021
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 54
IP  - 8
DP  - 2013 Aug 19
TI  - The effects of tenascin C knockdown on trabecular meshwork outflow resistance.
PG  - 5613-23
LID - 10.1167/iovs.13-11620 [doi]
AB  - PURPOSE: Tenascin C (TNC) is a matricellular glycoprotein whose expression in 
      adult tissue is indicative of tissue remodeling. The purpose of the current study 
      was to determine the localization of TNC in trabecular meshwork (TM) tissue and 
      to analyze the effects of TNC on intraocular pressure (IOP). METHODS: Human TM 
      frontal sections were immunostained with anti-TNC and imaged by confocal 
      microscopy. TNC mRNA and protein levels were quantitated in anterior segments 
      perfused at physiological and elevated pressure. Short, hairpin RNA (shRNA) 
      silencing lentivirus targeting full-length TNC (shTNC) was applied to anterior 
      segment perfusion organ cultures. The IOPs and central corneal thickness (CCT) of 
      wild-type, TNC(-/-), and tenascin X (TNX(-/-)) knockout mice were measured. 
      RESULTS: TNC was distributed in the juxtacanalicular (JCT) region of adult human 
      TM, predominantly in the basement membrane underlying the inner wall of Schlemm's 
      canal. Application of shTNC lentivirus to human and porcine anterior segments in 
      perfusion culture did not significantly affect outflow rate. Although TNC was 
      upregulated in response to pressure, there was no difference in outflow rate when 
      shTNC-silenced anterior segments were subjected to elevated pressure. 
      Furthermore, IOPs and CCTs were not significantly different between TNC(-/-) or 
      TNX(-/-) and wild-type mice. CONCLUSIONS: TNC does not appear to contribute 
      directly to outflow resistance. However, TNC immunolocalization in the JCT of 
      adult human eyes suggests that certain areas of the TM are being continuously 
      remodeled with or without an IOP increase.
FAU - Keller, Kate E
AU  - Keller KE
AD  - Casey Eye Institute, Oregon Health & Science University, Portland, Oregon 97239, 
      USA. gregorka@ohsu.edu
FAU - Vranka, Janice A
AU  - Vranka JA
FAU - Haddadin, Ramez I
AU  - Haddadin RI
FAU - Kang, Min-Hyung
AU  - Kang MH
FAU - Oh, Dong-Jin
AU  - Oh DJ
FAU - Rhee, Douglas J
AU  - Rhee DJ
FAU - Yang, Yong-Feng
AU  - Yang YF
FAU - Sun, Ying Ying
AU  - Sun YY
FAU - Kelley, Mary J
AU  - Kelley MJ
FAU - Acott, Ted S
AU  - Acott TS
LA  - eng
GR  - EY010572/EY/NEI NIH HHS/United States
GR  - EY003279/EY/NEI NIH HHS/United States
GR  - EY019654/EY/NEI NIH HHS/United States
GR  - R01 EY003279/EY/NEI NIH HHS/United States
GR  - R01 EY019654/EY/NEI NIH HHS/United States
GR  - R01 EY019643/EY/NEI NIH HHS/United States
GR  - EY008247/EY/NEI NIH HHS/United States
GR  - R01 EY008247/EY/NEI NIH HHS/United States
GR  - R01 EY021800/EY/NEI NIH HHS/United States
GR  - P30 EY010572/EY/NEI NIH HHS/United States
GR  - EY019643/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20130819
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Osteonectin)
RN  - 0 (RNA, Messenger)
RN  - 0 (SPARC protein, human)
RN  - 0 (Tenascin)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Anterior Eye Segment/physiology
MH  - Cadaver
MH  - Ciliary Body/physiology
MH  - Extracellular Matrix/metabolism
MH  - Gene Silencing
MH  - Homeostasis/physiology
MH  - Humans
MH  - Intraocular Pressure/*physiology
MH  - Lentivirus/genetics
MH  - Mice
MH  - Mice, Knockout
MH  - Organ Culture Techniques
MH  - Osteonectin/genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - Sclera/physiology
MH  - Swine
MH  - Tenascin/*genetics/*metabolism
MH  - Trabecular Meshwork/*physiology
MH  - Uvea/physiology
PMC - PMC3747717
OTO - NOTNLM
OT  - extracellular matrix
OT  - outflow resistance
OT  - tenascin C
OT  - trabecular meshwork
EDAT- 2013/07/25 06:00
MHDA- 2013/11/01 06:00
PMCR- 2013/08/01
CRDT- 2013/07/25 06:00
PHST- 2013/07/25 06:00 [entrez]
PHST- 2013/07/25 06:00 [pubmed]
PHST- 2013/11/01 06:00 [medline]
PHST- 2013/08/01 00:00 [pmc-release]
AID - iovs.13-11620 [pii]
AID - 10.1167/iovs.13-11620 [doi]
PST - epublish
SO  - Invest Ophthalmol Vis Sci. 2013 Aug 19;54(8):5613-23. doi: 10.1167/iovs.13-11620.