PMID- 23883798
OWN - NLM
STAT- MEDLINE
DCOM- 20131230
LR  - 20220408
IS  - 1945-8932 (Electronic)
IS  - 1945-8932 (Linking)
VI  - 27
IP  - 4
DP  - 2013 Jul-Aug
TI  - Relationship of eosinophils and plasma cells to biofilm in chronic 
      rhinosinusitis.
PG  - e85-90
LID - 10.2500/ajra.2013.27.3917 [doi]
AB  - BACKGROUND: This study investigates the relationship of eosinophils and plasma 
      cells to biofilm in chronic rhinosinusitis (CRS). A prospective observational 
      study was performed at the Keck Hospital, University of Southern California, 
      Department of Otolaryngology, Los Angeles, CA. METHODS: A total of 29 patients, 
      20 undergoing endoscopic sinus surgery for CRS and 9 control patients undergoing 
      septoplasty for nasal obstruction without history or evidence of CRS, were 
      included in this study. Contiguous sinonasal mucosa sample sections were examined 
      by hematoxylin and eosin (H&E), fluorescence in situ hybridization (FISH), and 
      immunohistochemistry (IHC) for biofilm, microbes, eosinophil major basic protein 
      (EMBP), and cluster designation 27 (CD27). EMBP and CD27 were used as eosinophil 
      and plasma cell markers, respectively. RESULTS: Biofilm was visualized in 15 of 
      20 patients with CRS on H&E sections, confirmed by microbial presence using FISH. 
      Biofilm was not identified in tissue samples of the nine control patients. On IHC 
      analysis, CD27 and EMBP expression were significantly higher in patients with CRS 
      compared with control (p < 0.05) and had greater expression in biofilm-positive 
      patients compared with biofilm-negative patients. Nasal polyps correlated with 
      higher expression of CD27 and EMBP, but in CRS patients without polyps CD27 and 
      EMBP was also significantly greater in biofilm-positive specimens compared with 
      biofilm-negative specimens. CONCLUSION: Biofilm presence in CRS appears to 
      correlate to host inflammatory response involving plasma cell and eosinophil 
      recruitment.
FAU - Arjomandi, Hamid
AU  - Arjomandi H
AD  - Department of Otolaryngology-Head and Neck Surgery, Keck School of Medicine, 
      University of Southern California, Los Angeles, CA 90031, USA.
FAU - Gilde, Jason
AU  - Gilde J
FAU - Zhu, Sutao
AU  - Zhu S
FAU - Delaney, Sean
AU  - Delaney S
FAU - Hochstim, Christian
AU  - Hochstim C
FAU - Mazhar, Kashif
AU  - Mazhar K
FAU - Wrobel, Bozena
AU  - Wrobel B
FAU - Markarian, Alexander
AU  - Markarian A
FAU - Masood, Rizwan
AU  - Masood R
FAU - Rice, Dale
AU  - Rice D
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Am J Rhinol Allergy
JT  - American journal of rhinology & allergy
JID - 101490775
RN  - 0 (Biomarkers)
RN  - 0 (Immunologic Factors)
RN  - 0 (Tumor Necrosis Factor Receptor Superfamily, Member 7)
RN  - EC 3.1.27.- (Eosinophil Major Basic Protein)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Biofilms/*growth & development
MH  - Biomarkers/metabolism
MH  - Chronic Disease
MH  - Eosinophil Major Basic Protein/*genetics
MH  - Eosinophils/*metabolism
MH  - Female
MH  - Hospitals, University
MH  - Humans
MH  - Immunologic Factors/genetics
MH  - Male
MH  - Middle Aged
MH  - Nasal Mucosa/metabolism
MH  - Nasal Septum/surgery
MH  - Plasma Cells/*metabolism
MH  - Prospective Studies
MH  - *Rhinitis/diagnosis/genetics/metabolism/surgery
MH  - *Sinusitis/diagnosis/genetics/metabolism/surgery
MH  - Tumor Necrosis Factor Receptor Superfamily, Member 7/*genetics
EDAT- 2013/07/26 06:00
MHDA- 2014/01/01 06:00
CRDT- 2013/07/26 06:00
PHST- 2013/07/26 06:00 [entrez]
PHST- 2013/07/26 06:00 [pubmed]
PHST- 2014/01/01 06:00 [medline]
AID - 10.2500/ajra.2013.27.3917 [doi]
PST - ppublish
SO  - Am J Rhinol Allergy. 2013 Jul-Aug;27(4):e85-90. doi: 10.2500/ajra.2013.27.3917.