PMID- 23883899
OWN - NLM
STAT- MEDLINE
DCOM- 20131025
LR  - 20130725
IS  - 2163-0763 (Electronic)
IS  - 2163-0755 (Linking)
VI  - 75
IP  - 2 Suppl 2
DP  - 2013 Aug
TI  - Intramuscular transplantation and survival of freshly isolated bone marrow cells 
      following skeletal muscle ischemia-reperfusion injury.
PG  - S142-9
LID - 10.1097/TA.0b013e31829ac1fa [doi]
AB  - BACKGROUND: Delayed treatment cellular therapies offer an attractive means to 
      treat extremity injuries involving acute skeletal muscle ischemia-reperfusion 
      injury (I/R). Bone marrow is a rich source of stem and progenitor cells with the 
      potential to improve skeletal muscle regeneration. The extent to which bone 
      marrow cells (BMCs) may be useful for I/R is not known. The purposes of this 
      study were twofold: (1) to evaluate BMC survival following intramuscular 
      injection 0, 2, 7, and 14 days after injury and (2) to determine whether BMCs 
      improve functional recovery following I/R. METHODS: Magnetic-activated cell 
      sorting was used to isolate lineage-negative (Lin(-)) BMCs and enrich for stem and 
      progenitor cells. To evaluate in vivo cell survival following I/R, Lin(-) BMCs were 
      injected intramuscularly 0, 2, 7, and 14 days after I/R, and bioluminescent 
      imaging was performed for up to 28 days after cell injections. To assess their 
      ability to improve muscle regeneration, intramuscular injections were performed 2 
      days after injury, and in vivo muscle function was assessed 14 days later. 
      RESULTS: Lin(-) BMCs survived throughout the study period regardless of the timing 
      of delivery. Intramuscular injection of Lin(-) BMCs did not improve maximal 
      isometric torque (300 Hz); however, both saline-injected and Lin(-) BMC-injected 
      muscles exhibited an increase in the twitch-tetanus ratio, suggesting that damage 
      incurred with the intramuscular injections may have had deleterious consequences 
      for functional recovery. CONCLUSION: Although BMCs injected intramuscularly 
      survived cell transplantation, they failed to improve muscle function following 
      I/R. The ability of BMCs to persist in injured muscle following I/R lends to the 
      possibility that with further development, their full potential can be realized.
FAU - Corona, Benjamin T
AU  - Corona BT
AD  - Extremity Trauma and Regenerative Medicine Task Area, US Army Institute of 
      Surgical Research, Fort Sam Houston, Texas, USA.
FAU - Wenke, Joseph C
AU  - Wenke JC
FAU - Walters, Thomas J
AU  - Walters TJ
FAU - Rathbone, Christopher R
AU  - Rathbone CR
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Trauma Acute Care Surg
JT  - The journal of trauma and acute care surgery
JID - 101570622
SB  - IM
MH  - Animals
MH  - Bone Marrow Transplantation/*methods
MH  - Cell Survival
MH  - Injections, Intramuscular
MH  - Luminescent Measurements
MH  - Mice
MH  - Mice, Transgenic
MH  - Muscle, Skeletal/*injuries/physiopathology
MH  - Reperfusion Injury/*therapy
EDAT- 2013/08/02 06:00
MHDA- 2013/10/26 06:00
CRDT- 2013/07/26 06:00
PHST- 2013/07/26 06:00 [entrez]
PHST- 2013/08/02 06:00 [pubmed]
PHST- 2013/10/26 06:00 [medline]
AID - 01586154-201308002-00008 [pii]
AID - 10.1097/TA.0b013e31829ac1fa [doi]
PST - ppublish
SO  - J Trauma Acute Care Surg. 2013 Aug;75(2 Suppl 2):S142-9. doi: 
      10.1097/TA.0b013e31829ac1fa.