PMID- 23884567
OWN - NLM
STAT- MEDLINE
DCOM- 20140811
LR  - 20211021
IS  - 1573-904X (Electronic)
IS  - 0724-8741 (Linking)
VI  - 31
IP  - 1
DP  - 2014 Jan
TI  - Modulation of polyplex release from biodegradable microparticles through 
      poly(ethylenimine) modification and varying loading concentration.
PG  - 77-85
LID - 10.1007/s11095-013-1133-1 [doi]
AB  - PURPOSE: This work investigates the effects of hyaluronic acid (HA) conjugated 
      onto branched poly(ethylenimine) (bPEI) and varying loading concentrations of 
      these polymers complexed with DNA on their release from 
      poly(DL-lactic-co-glycolic acid) (PLGA) microparticles and the transfection of 
      target cells. METHODS: To examine the effect of alteration of the gene delivery 
      polymer on the system, we observed the morphology, size, loading efficiency, 
      polymer and DNA release, and the transfection efficiency for the microparticles 
      formed with three internal phase loading concentrations during microparticle 
      formation. RESULTS: Addition of HA to this vector allowed for increased loading 
      concentration within these systems and significantly altered release kinetics 
      without changing the morphology of the particles. The incorporation of HA onto 
      the bPEI backbone significantly increased the transfection efficiency of the 
      complexes released from the corresponding microparticle formulation. CONCLUSIONS: 
      The results show that the modification of bPEI with HA and the concentration of 
      loaded polymer/DNA complexes can significantly alter the entrapment and release 
      profiles from PLGA microparticles. This is significant in that it offers insight 
      into the effects of modification of gene delivery vectors on a controlled release 
      system designed to achieve a sustained therapeutic response.
FAU - Needham, Clark J
AU  - Needham CJ
AD  - Department of Bioengineering, Rice University, 6500 Main St, Houston, Texas, 
      77030, USA.
FAU - Shah, Sarita R
AU  - Shah SR
FAU - Mountziaris, Paschalia M
AU  - Mountziaris PM
FAU - Kasper, F Kurtis
AU  - Kasper FK
FAU - Mikos, Antonios G
AU  - Mikos AG
LA  - eng
GR  - R01 AR57083/AR/NIAMS NIH HHS/United States
GR  - R21 AR56076/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20130725
PL  - United States
TA  - Pharm Res
JT  - Pharmaceutical research
JID - 8406521
RN  - 0 (Aziridines)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Polymers)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 54P5FEX9FH (aziridine)
RN  - 9004-61-9 (Hyaluronic Acid)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Aziridines/*chemistry/metabolism
MH  - Biocompatible Materials/*chemistry/metabolism
MH  - Cells, Cultured
MH  - Chemistry, Pharmaceutical/methods
MH  - DNA/chemistry
MH  - Fibroblasts/metabolism
MH  - Gene Transfer Techniques
MH  - Hyaluronic Acid/*chemistry/metabolism
MH  - Lactic Acid/chemistry/metabolism
MH  - Microspheres
MH  - Particle Size
MH  - Polyglycolic Acid/chemistry/metabolism
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers/*chemistry/metabolism
MH  - Rats
MH  - Transfection
EDAT- 2013/07/26 06:00
MHDA- 2014/08/12 06:00
CRDT- 2013/07/26 06:00
PHST- 2013/04/30 00:00 [received]
PHST- 2013/06/24 00:00 [accepted]
PHST- 2013/07/26 06:00 [entrez]
PHST- 2013/07/26 06:00 [pubmed]
PHST- 2014/08/12 06:00 [medline]
AID - 10.1007/s11095-013-1133-1 [doi]
PST - ppublish
SO  - Pharm Res. 2014 Jan;31(1):77-85. doi: 10.1007/s11095-013-1133-1. Epub 2013 Jul 
      25.