PMID- 23884820
OWN - NLM
STAT- MEDLINE
DCOM- 20150402
LR  - 20130725
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 17
IP  - 15
DP  - 2013
TI  - Effect of HMGB1/NF-kappaB in hyperbaric oxygen treatment on decreasing injury caused 
      by skin flap grafts in rats.
PG  - 2010-8
LID - 4803 [pii]
AB  - BACKGROUND: Skin flap grafting (SFG) is a common surgical operation, and 
      hyperbaric oxygen treatment (HBOT) is an important strategy for restoring the 
      grafted skin flap. Thus, we employed a rat skin flap grafting model treated with 
      HBO, and expression levels of high mobility group protein 1 (HMGB1) and NF-kappaB 
      (nuclear factor-kappaB) were characterized. MATERIALS AND METHODS: Forty rats 
      were randomly assigned to 5 groups: (1) sham-operation (SH), (2) ischemia 
      followed by reperfusion 3 days after operation (IR3d), (3) ischemia followed by 
      reperfusion 5 days after operation (IR5d), (4) ischemia followed by reperfusion 
      and HBOT 3 days after operation (HBO3d), and (5) ischemia followed by reperfusion 
      and HBOT 5 days after operation (HBO5d). Elevated pedicled skin flaps were 
      designed (size, 9 cm x 6 cm), and feeding vessels were clamped. The microvascular 
      clamp was removed 3 h later and flow was restored. In the HBO3d and HBO5d groups, 
      rats received 1 h of hyperbaric oxygen (HBO) starting immediately after surgery 
      for 3 days and 5 days, respectively. Upon completion of animal experiments, rats 
      were euthanized by general anesthesia, and blood samples were taken for testing. 
      The tissues were sectioned for western blotting and immunohistochemical staining. 
      RESULTS: Expression of HMGB1 and NF-kappaB proteins in the HBO groups was lower than 
      in the IR groups. CONCLUSIONS: The results suggest that HBOT can be used to 
      reduce ischemia-reperfusion (IR) injury of skin flap grafts.
FAU - Liang, F
AU  - Liang F
AD  - Department of Hyperbaric Oxygen, Beijing Chaoyang Hospital, Capital Medical 
      University, Beijing, China. 13322813330@189.cn  
FAU - Kang, N
AU  - Kang N
FAU - Liu, X
AU  - Liu X
FAU - Yang, J
AU  - Yang J
FAU - Li, Z
AU  - Li Z
FAU - Tan, J-W
AU  - Tan JW
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (HMGB1 Protein)
RN  - 0 (Hbp1 protein, rat)
RN  - 0 (NF-kappa B)
SB  - IM
MH  - Animals
MH  - Graft Survival
MH  - HMGB1 Protein/antagonists & inhibitors/*metabolism
MH  - *Hyperbaric Oxygenation
MH  - Male
MH  - NF-kappa B/*metabolism
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/*metabolism/pathology/*therapy
MH  - Skin/*metabolism/pathology
MH  - Skin Transplantation
EDAT- 2013/07/26 06:00
MHDA- 2015/04/04 06:00
CRDT- 2013/07/26 06:00
PHST- 2013/07/26 06:00 [entrez]
PHST- 2013/07/26 06:00 [pubmed]
PHST- 2015/04/04 06:00 [medline]
AID - 4803 [pii]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2013;17(15):2010-8.