PMID- 23885671
OWN - NLM
STAT- MEDLINE
DCOM- 20140213
LR  - 20130802
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1304
DP  - 2013 Aug 23
TI  - High-speed gradient separations of peptides and proteins using polymer-monolithic 
      poly(styrene-co-divinylbenzene) capillary columns at ultra-high pressure.
PG  - 177-82
LID - S0021-9673(13)01084-4 [pii]
LID - 10.1016/j.chroma.2013.07.040 [doi]
AB  - For the first time, polymer monolithic capillary columns have been employed at 
      ultra-high-pressure liquid chromatographic conditions (UHPLC) to investigate 
      their potential for high-speed separations of peptides and intact proteins. In 
      comparison to conventional flow rates and gradient conditions, a substantial 
      decrease in analysis time (>factor 4) can be achieved when operating monolithic 
      columns such as ultra-high-pressure conditions while scaling the gradient volume. 
      The effects of flow rate and column length on the peak capacity and the gradient 
      performance limits were assessed for the separation of peptide and protein 
      mixtures applying the maximum system pressure (80MPa) and a fixed gradient 
      steepness. The potential for ultra-fast gradient separations of large 
      biomolecules was further demonstrated for very steep gradients (gradient 
      times<<1min). A tryptic digest of cytochrome c was separated using a gradient time 
      of only 1min. Finally, the run-to-run repeatability and column robustness were 
      assessed at ultra-high pressure conditions (after>800 runs) with consecutive 
      steep 1min separations of peptides, yielding RSD values below 0.12% in retention 
      time.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Vaast, Axel
AU  - Vaast A
AD  - Vrije Universiteit Brussel, Department of Chemical Engineering, Pleinlaan 2, 
      B-1050 Brussels, Belgium.
FAU - Novakova, Lucie
AU  - Novakova L
FAU - Desmet, Gert
AU  - Desmet G
FAU - de Haan, Bjorn
AU  - de Haan B
FAU - Swart, Remco
AU  - Swart R
FAU - Eeltink, Sebastiaan
AU  - Eeltink S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130713
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Peptides)
RN  - 0 (Polymers)
RN  - 0 (Polystyrenes)
RN  - 0 (Proteins)
RN  - 0 (divinylbenzene-polystyrene copolymer)
RN  - 9007-43-6 (Cytochromes c)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Chickens
MH  - Chromatography, High Pressure Liquid/economics/*methods
MH  - Cytochromes c/isolation & purification
MH  - Horses
MH  - Peptides/*isolation & purification
MH  - Polymers/chemistry
MH  - Polystyrenes/*chemistry
MH  - Proteins/*isolation & purification
MH  - Proteomics/economics/methods
MH  - Time Factors
OTO - NOTNLM
OT  - Capillary columns
OT  - Proteomics
OT  - Repeatability
OT  - Ultra-fast separation
OT  - Ultra-high pressure
EDAT- 2013/07/28 06:00
MHDA- 2014/02/14 06:00
CRDT- 2013/07/27 06:00
PHST- 2013/07/05 00:00 [received]
PHST- 2013/07/10 00:00 [accepted]
PHST- 2013/07/27 06:00 [entrez]
PHST- 2013/07/28 06:00 [pubmed]
PHST- 2014/02/14 06:00 [medline]
AID - S0021-9673(13)01084-4 [pii]
AID - 10.1016/j.chroma.2013.07.040 [doi]
PST - ppublish
SO  - J Chromatogr A. 2013 Aug 23;1304:177-82. doi: 10.1016/j.chroma.2013.07.040. Epub 
      2013 Jul 13.