PMID- 23885864
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130729
LR  - 20130726
IS  - 1354-750X (Print)
IS  - 1354-750X (Linking)
VI  - 4
IP  - 3
DP  - 1999
TI  - Induction of cytochrome P450 1A and DNA damage in isolated rainbow trout 
      (Oncorhynchus mykiss) hepatocytes by 2,3,7,8 tetrachlorodibenzo-p-dioxin.
PG  - 214-28
LID - 10.1080/135475099230886 [doi]
AB  - Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on hepatocytes isolated 
      from immature rainbow trout (Oncorhynchus mykiss) by collagenase perfusion were 
      investigated with respect to induction of cytochrome P450 1A (CYP1A) enzyme 
      activities and protein contents as well as DNA damage. Exposure of primary 
      rainbow trout hepatocytes to TCDD resulted in increased CYP1A contents, as 
      determined by immunoblotting, enhanced activities of 
      7-ethoxyresorufin-O-deethylase (EROD) and increased DNA damage as determined by 
      the comet assay. By means of electron microscopy, no symptoms of cytotoxicity 
      could be observed except for slight increases of lysosomal components and the 
      smooth endoplasmic reticulum. Whereas CYP1A contents constantly increased over 
      the duration of the entire experiment, EROD activities remained constant from day 
      3 of exposure to 1 nM TCDD; maximum induction of CYP1A activities was reached 
      with 0.1 nM TCDD after 5 days. DNA damage increased in a time- and dose-dependent 
      fashion until day 3. After 5 days, DNA damage was less pronounced, and the number 
      of damaged nuclei declined in all TCDD concentrations. Since TCDD has been shown 
      to not directly react with DNA, metabolism of TCDD or TCDD-induced changes in 
      other metabolic pathways are suspected to result in the production of 
      DNA-reactive (endogenous) substances.
FAU - T Berbner N Rahman T Braunbeck
AU  - T Berbner N Rahman T Braunbeck
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biomarkers
JT  - Biomarkers : biochemical indicators of exposure, response, and susceptibility to 
      chemicals
JID - 9606000
EDAT- 1999/01/01 00:00
MHDA- 1999/01/01 00:01
CRDT- 2013/07/27 06:00
PHST- 2013/07/27 06:00 [entrez]
PHST- 1999/01/01 00:00 [pubmed]
PHST- 1999/01/01 00:01 [medline]
AID - 10.1080/135475099230886 [doi]
PST - ppublish
SO  - Biomarkers. 1999;4(3):214-28. doi: 10.1080/135475099230886.