PMID- 23885956
OWN - NLM
STAT- MEDLINE
DCOM- 20140131
LR  - 20161125
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 61
IP  - 33
DP  - 2013 Aug 21
TI  - Transepithelial permeability studies of flavan-3-ol-C-glucosides and procyanidin 
      dimers and trimers across the Caco-2 cell monolayer.
PG  - 7932-40
LID - 10.1021/jf402019f [doi]
AB  - In this study the permeability of two flavanol-C-glucosides (FCglcs) and five 
      dimeric and trimeric flavan-3-ols, namely, procyanidins (PCs), was investigated 
      with the human colon carcinoma cell line (Caco-2) model. These compounds can be 
      found especially in cocoa, and they are of great interest due to their assumed 
      beneficial health effects. Transepithelial electrical resistance (TEER) and 
      capacitance were measured online with a CellZscope device prior to and during the 
      whole experiment to guarantee the maintenance of the barrier properties. The 
      transport experiments with pure, single compounds (50-300 muM) from apical to 
      basolateral side showed slight permeation of PCs A2, B2, and B5 and cinnamtannin 
      B1 (CB1) as well as (-)-catechin-6-C-glucoside (C6Cglc) and 
      (-)-catechin-8-C-glucoside (C8Cglc) of about 0.02-0.2% after 24 h. Transport of 
      PC C1 could not be detected. Inhibition of P-glycoprotein (Pgp) increased the 
      permeation of PC B2 and CB1 to the basolateral side, which indicates that Pgp 
      counteracts the transport of these compounds. Metabolites (epicatechin, 3'- and 
      4'-O-epicatechin) in very small amounts were detectable only for PC B2. These are 
      the first data concerning the permeability of flavan-3-ol-C-glucosides across the 
      Caco-2 cell monolayer.
FAU - Hemmersbach, Sarah
AU  - Hemmersbach S
AD  - Institute of Food Chemistry, Westfalische Wilhelms-Universitat Munster, Munster, 
      Germany.
FAU - Brauer, Susanne S
AU  - Brauer SS
FAU - Huwel, Sabine
AU  - Huwel S
FAU - Galla, Hans-Joachim
AU  - Galla HJ
FAU - Humpf, Hans-Ulrich
AU  - Humpf HU
LA  - eng
PT  - Journal Article
DEP - 20130808
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Biflavonoids)
RN  - 0 (Flavonoids)
RN  - 0 (Glucosides)
RN  - 0 (Plant Extracts)
RN  - 0 (Proanthocyanidins)
RN  - 35HDD3NRIE (flavan-3-ol)
RN  - 4852-22-6 (procyanidin)
RN  - 8R1V1STN48 (Catechin)
SB  - IM
MH  - Biflavonoids/chemistry/*metabolism
MH  - Caco-2 Cells
MH  - Catechin/chemistry/*metabolism
MH  - Dimerization
MH  - Epithelial Cells/*metabolism
MH  - Flavonoids/*metabolism
MH  - Glucosides/chemistry/*metabolism
MH  - Humans
MH  - Molecular Structure
MH  - Permeability
MH  - Plant Extracts/chemistry/*metabolism
MH  - Proanthocyanidins/chemistry/*metabolism
EDAT- 2013/07/28 06:00
MHDA- 2014/02/01 06:00
CRDT- 2013/07/27 06:00
PHST- 2013/07/27 06:00 [entrez]
PHST- 2013/07/28 06:00 [pubmed]
PHST- 2014/02/01 06:00 [medline]
AID - 10.1021/jf402019f [doi]
PST - ppublish
SO  - J Agric Food Chem. 2013 Aug 21;61(33):7932-40. doi: 10.1021/jf402019f. Epub 2013 
      Aug 8.