PMID- 2388627
OWN - NLM
STAT- MEDLINE
DCOM- 19900926
LR  - 20210526
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 10
IP  - 9
DP  - 1990 Sep
TI  - Brain and muscle creatine kinase genes contain common TA-rich recognition 
      protein-binding regulatory elements.
PG  - 4826-36
AB  - We have previously reported that the rat brain creatine kinase (ckb) gene 
      promoter contains an AT-rich sequence that is a binding site for a protein called 
      TARP (TA-rich recognition protein). This AT-rich segment is a positively acting 
      regulatory element for the ckb promoter. A similar AT-rich DNA segment is found 
      at the 3' end of the 5' muscle-specific enhancer of the rat muscle creatine 
      kinase (ckm) gene and has been shown to be necessary for full muscle-specific 
      enhancer activity. In this report, we show that TARP binds not only to the ckb 
      promoter but also to the AT-rich segment at the 3' end of the muscle-specific ckm 
      enhancer. A second, weaker TARP-binding site was identified in the ckm enhancer 
      and lies at the 5' end of the minimal enhancer segment. TARP was found in both 
      muscle cells (C2 and L6 myotubes) and nonmuscle (HeLa) cells and appeared to be 
      indistinguishable from both sources, as judged by gel retardation and 
      footprinting assays. The TARP-binding sites in the ckm enhancer and the ckb 
      promoter were found to be functionally interchangeable. We propose that TARP is 
      active in both muscle and nonmuscle cells and that it is one of many potential 
      activators that may interact with muscle-specific regulators to determine the 
      myogenic phenotype.
FAU - Horlick, R A
AU  - Horlick RA
AD  - E. I. du Pont de Nemours and Co., Inc., Wilmington, Delaware 19880-0328.
FAU - Hobson, G M
AU  - Hobson GM
FAU - Patterson, J H
AU  - Patterson JH
FAU - Mitchell, M T
AU  - Mitchell MT
FAU - Benfield, P A
AU  - Benfield PA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Oligonucleotide Probes)
RN  - EC 2.7.3.2 (Creatine Kinase)
RN  - EC 3.1.21.1 (Deoxyribonuclease I)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Brain/*enzymology
MH  - Creatine Kinase/*genetics
MH  - Deoxyribonuclease I
MH  - *Genes
MH  - HeLa Cells/enzymology
MH  - Humans
MH  - Molecular Sequence Data
MH  - Muscles/*enzymology
MH  - Nucleotide Mapping
MH  - Oligonucleotide Probes
MH  - Promoter Regions, Genetic
MH  - Rats
MH  - Sequence Homology, Nucleic Acid
MH  - Transcription, Genetic
MH  - Transfection
PMC - PMC361091
EDAT- 1990/09/01 00:00
MHDA- 1990/09/01 00:01
PMCR- 1990/09/01
CRDT- 1990/09/01 00:00
PHST- 1990/09/01 00:00 [pubmed]
PHST- 1990/09/01 00:01 [medline]
PHST- 1990/09/01 00:00 [entrez]
PHST- 1990/09/01 00:00 [pmc-release]
AID - 10.1128/mcb.10.9.4826-4836.1990 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1990 Sep;10(9):4826-36. doi: 10.1128/mcb.10.9.4826-4836.1990.