PMID- 23886622
OWN - NLM
STAT- MEDLINE
DCOM- 20140407
LR  - 20211021
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 163
IP  - 1
DP  - 2013 Sep
TI  - Complementation of HYPONASTIC LEAVES1 by double-strand RNA-binding domains of 
      DICER-LIKE1 in nuclear dicing bodies.
PG  - 108-17
LID - 10.1104/pp.113.219071 [doi]
AB  - MicroRNAs (miRNAs) are a class of small regulatory RNAs that are found in almost 
      all of the eukaryotes. Arabidopsis (Arabidopsis thaliana) miRNAs are processed 
      from primary miRNAs (pri-miRNAs), mainly by the ribonuclease III-like enzyme 
      DICER-LIKE1 (DCL1) and its specific partner, HYPONASTIC LEAVES1 (HYL1), a 
      double-strand RNA-binding protein, both of which contain two double-strand 
      RNA-binding domains (dsRBDs). These dsRBDs are essential for miRNA processing, 
      but the functions of them are not clear. Here, we report that the two dsRBDs of 
      DCL1 (DCL1-D1D2), and to some extent the second dsRBD (DCL1-D2), complement the 
      hyl1 mutant, but not the first dsRBD of DCL1 (DCL1-D1). DCL1-D1 is diffusely 
      distributed throughout the nucleoplasm, whereas DCL1-D2 and DCL1-D1D2 concentrate 
      in nuclear dicing bodies in which DCL1 and HYL1 colocalize. We show further that 
      protein-protein interaction is mainly mediated by DCL1-D2, while DCL1-D1 plays a 
      major role in binding of pri-miRNAs. These results suggest parallel roles between 
      C-terminal dsRBDs of DCL1 and N-terminal dsRBDs of HYL1 and support a model in 
      which Arabidopsis pri-miRNAs are recruited to dicing bodies through functionally 
      divergent dsRBDs of microprocessor for accurate processing of plant pri-miRNAs.
FAU - Liu, Qi
AU  - Liu Q
AD  - National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant 
      Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese 
      Academy of Sciences, Shanghai 200032, China.
FAU - Yan, Qingqing
AU  - Yan Q
FAU - Liu, Yin
AU  - Liu Y
FAU - Hong, Fang
AU  - Hong F
FAU - Sun, Zhenfei
AU  - Sun Z
FAU - Shi, Leilei
AU  - Shi L
FAU - Huang, Ying
AU  - Huang Y
FAU - Fang, Yuda
AU  - Fang Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130725
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (HYL1 protein, Arabidopsis)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA-Binding Proteins)
RN  - EC 3.1.26.3 (DCL1 protein, Arabidopsis)
RN  - EC 3.1.26.3 (Ribonuclease III)
SB  - IM
MH  - Arabidopsis/*genetics
MH  - Arabidopsis Proteins/analysis/*genetics/metabolism
MH  - Binding Sites
MH  - Cell Cycle Proteins/analysis/*genetics/metabolism
MH  - MicroRNAs
MH  - RNA-Binding Proteins/*genetics
MH  - Ribonuclease III/analysis/*genetics/metabolism
PMC - PMC3762634
EDAT- 2013/07/28 06:00
MHDA- 2014/04/08 06:00
PMCR- 2013/07/25
CRDT- 2013/07/27 06:00
PHST- 2013/07/27 06:00 [entrez]
PHST- 2013/07/28 06:00 [pubmed]
PHST- 2014/04/08 06:00 [medline]
PHST- 2013/07/25 00:00 [pmc-release]
AID - pp.113.219071 [pii]
AID - 219071 [pii]
AID - 10.1104/pp.113.219071 [doi]
PST - ppublish
SO  - Plant Physiol. 2013 Sep;163(1):108-17. doi: 10.1104/pp.113.219071. Epub 2013 Jul 
      25.