PMID- 23887300
OWN - NLM
STAT- MEDLINE
DCOM- 20140929
LR  - 20230210
IS  - 1530-0285 (Electronic)
IS  - 0893-3952 (Linking)
VI  - 27
IP  - 1
DP  - 2014 Jan
TI  - EGFR alterations and EML4-ALK rearrangement in primary adenocarcinoma of the 
      urinary bladder.
PG  - 107-12
LID - 10.1038/modpathol.2013.132 [doi]
AB  - The identification of mutations in epidermal growth factor receptor (EGFR) and 
      translocations involving anaplastic lymphoma kinase (ALK) in lung adenocarcinoma 
      has drastically changed understanding of the disease and led to the development 
      of targeted therapies. Adenocarcinoma of the urinary bladder is rare and poorly 
      understood at the molecular level. We undertook this study to determine whether 
      EGFR mutations, increases in EGFR copy number, or ALK translocations are present 
      in these tumors. Twenty-eight cases of primary bladder adenocarcinoma were 
      analyzed. For EGFR mutational analysis, PCR-amplified products were analyzed on 
      the Q24 Pyrosequencer with Qiagen EGFR Pyro Kits. All cases were analyzed via 
      fluorescence in situ hybridization (FISH) using Vysis ALK Break Apart FISH Probes 
      for detection of ALK chromosomal translocation and Vysis Dual Color Probes to 
      assess for increased gene copy number of EGFR. None of the 28 cases examined 
      showed mutational events in EGFR or ALK rearrangements. EGFR polysomy was seen in 
      10 out of 28 (36%) cases. No correlation with EGFR polysomy was seen in the 
      tumors with respect to age, histologic subtypes, pathologic stage, or lymph node 
      metastasis. In summary, EGFR mutations and ALK rearrangements do not appear to be 
      involved in the development of primary adenocarcinoma of the urinary bladder. A 
      subgroup of cases (36%), however, demonstrated increased gene copy number of EGFR 
      by FISH.
FAU - Alexander, Riley E
AU  - Alexander RE
AD  - Department of Pathology and Laboratory Medicine, Indiana University School of 
      Medicine, Indianapolis, IN, USA.
FAU - Montironi, Rodolfo
AU  - Montironi R
AD  - Institute of Pathological Anatomy and Histopathology, School of Medicine, 
      Polytechnic University of the Marche Region (Ancona), United Hospitals, Ancona, 
      Italy.
FAU - Lopez-Beltran, Antonio
AU  - Lopez-Beltran A
AD  - Department of Pathology, Cordoba University, Cordoba, Spain.
FAU - Williamson, Sean R
AU  - Williamson SR
AD  - Department of Pathology and Laboratory Medicine, Indiana University School of 
      Medicine, Indianapolis, IN, USA.
FAU - Wang, Mingsheng
AU  - Wang M
AD  - Department of Pathology and Laboratory Medicine, Indiana University School of 
      Medicine, Indianapolis, IN, USA.
FAU - Post, Kristin M
AU  - Post KM
AD  - Department of Pathology and Laboratory Medicine, Indiana University School of 
      Medicine, Indianapolis, IN, USA.
FAU - Sen, Joyashree D
AU  - Sen JD
AD  - Department of Pathology and Laboratory Medicine, Indiana University School of 
      Medicine, Indianapolis, IN, USA.
FAU - Arnold, Ashley K
AU  - Arnold AK
AD  - Department of Pathology and Laboratory Medicine, Indiana University School of 
      Medicine, Indianapolis, IN, USA.
FAU - Zhang, Shaobo
AU  - Zhang S
AD  - Department of Pathology and Laboratory Medicine, Indiana University School of 
      Medicine, Indianapolis, IN, USA.
FAU - Wang, Xiaoyan
AU  - Wang X
AD  - Department of Pathology and Laboratory Medicine, Indiana University School of 
      Medicine, Indianapolis, IN, USA.
FAU - Koch, Michael O
AU  - Koch MO
AD  - Department of Urology, Indiana University School of Medicine, Indianapolis, IN, 
      USA.
FAU - Hahn, Noah M
AU  - Hahn NM
AD  - Department of Internal Medicine, Indiana University School of Medicine, 
      Indianapolis, IN, USA.
FAU - Masterson, Timothy A
AU  - Masterson TA
AD  - Department of Urology, Indiana University School of Medicine, Indianapolis, IN, 
      USA.
FAU - MacLennan, Gregory T
AU  - MacLennan GT
AD  - Department of Pathology, Case Western Reserve University, Cleveland, OH, USA.
FAU - Davidson, Darrell D
AU  - Davidson DD
AD  - Department of Pathology and Laboratory Medicine, Indiana University School of 
      Medicine, Indianapolis, IN, USA.
FAU - Comperat, Eva
AU  - Comperat E
AD  - Department of Pathology, Groupe Hospitalier Pitie-Salpetriere, Paris, France.
FAU - Cheng, Liang
AU  - Cheng L
AD  - 1] Department of Pathology and Laboratory Medicine, Indiana University School of 
      Medicine, Indianapolis, IN, USA [2] Department of Urology, Indiana University 
      School of Medicine, Indianapolis, IN, USA.
LA  - eng
PT  - Journal Article
DEP - 20130726
PL  - United States
TA  - Mod Pathol
JT  - Modern pathology : an official journal of the United States and Canadian Academy 
      of Pathology, Inc
JID - 8806605
RN  - 0 (EML4-ALK fusion protein, human)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - EC 2.7.10.1 (EGFR protein, human)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Adenocarcinoma/enzymology/*genetics/pathology
MH  - DNA Mutational Analysis
MH  - ErbB Receptors/*genetics
MH  - Gene Dosage
MH  - *Gene Rearrangement
MH  - Genetic Predisposition to Disease
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Mutation
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Phenotype
MH  - Polymerase Chain Reaction
MH  - Risk Factors
MH  - Urinary Bladder Neoplasms/enzymology/*genetics/pathology
EDAT- 2013/07/28 06:00
MHDA- 2014/09/30 06:00
CRDT- 2013/07/27 06:00
PHST- 2013/03/15 00:00 [received]
PHST- 2013/06/06 00:00 [revised]
PHST- 2013/06/06 00:00 [accepted]
PHST- 2013/07/27 06:00 [entrez]
PHST- 2013/07/28 06:00 [pubmed]
PHST- 2014/09/30 06:00 [medline]
AID - S0893-3952(22)03604-3 [pii]
AID - 10.1038/modpathol.2013.132 [doi]
PST - ppublish
SO  - Mod Pathol. 2014 Jan;27(1):107-12. doi: 10.1038/modpathol.2013.132. Epub 2013 Jul 
      26.