PMID- 23887804
OWN - NLM
STAT- MEDLINE
DCOM- 20131105
LR  - 20220309
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 54
IP  - 9
DP  - 2013 Sep 5
TI  - Optical coherence tomography study of experimental anterior ischemic optic 
      neuropathy and histologic confirmation.
PG  - 5981-8
LID - 10.1167/iovs.13-12419 [doi]
AB  - PURPOSE: The optic nerve is part of the central nervous system, and interruption 
      of this pathway due to ischemia typically results in optic atrophy and loss of 
      retinal ganglion cells. In this study, we assessed in vivo retinal changes 
      following murine anterior ischemic optic neuropathy (AION) by using 
      spectral-domain optical coherence tomography (SD-OCT) and compared these anatomic 
      measurements to that of histology. METHODS: We induced ischemia at the optic disc 
      via laser-activated photochemical thrombosis, performed serial SD-OCT and manual 
      segmentation of the retinal layers to measure the ganglion cell complex (GCC) and 
      total retinal thickness, and correlated these measurements with that of 
      histology. RESULTS: There was impaired perfusion and leakage at the optic disc on 
      fluorescein angiography immediately after AION and severe swelling and distortion 
      of the peripapillary retina on day-1. We used SD-OCT to quantify the changes in 
      retinal thickness following experimental AION, which revealed significant 
      thickening of the GCC on day-1 after ischemia followed by gradual thinning that 
      plateaued by week-3. Thickness of the peripapillary sensory retina was also 
      increased on day-1 and thinned chronically. This pattern of acute retinal 
      swelling and chronic thinning on SD-OCT correlated well with changes seen in 
      histology and corresponded to loss of retinal ganglion layer cells after 
      ischemia. CONCLUSIONS: This was a serial SD-OCT quantification of acute and 
      chronic changes following experimental AION, which revealed changes in the GCC 
      similar to that of human AION, but over a time frame of weeks rather than months.
FAU - Ho, Joyce K
AU  - Ho JK
AD  - Department of Ophthalmology, Stanford University School of Medicine, Stanford, 
      California, USA.
FAU - Stanford, Madison P
AU  - Stanford MP
FAU - Shariati, Mohammad A
AU  - Shariati MA
FAU - Dalal, Roopa
AU  - Dalal R
FAU - Liao, Yaping Joyce
AU  - Liao YJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130905
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Fluorescein Angiography
MH  - Fundus Oculi
MH  - Lasers/adverse effects
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Optic Disk/*pathology
MH  - Optic Neuropathy, Ischemic/etiology/*pathology
MH  - Reproducibility of Results
MH  - Retinal Ganglion Cells/*pathology
MH  - Severity of Illness Index
MH  - Tomography, Optical Coherence/*methods
PMC - PMC3771554
OTO - NOTNLM
OT  - AION
OT  - SD-OCT
OT  - animal model
OT  - optic neuropathy
OT  - retinal ganglion cell
EDAT- 2013/07/28 06:00
MHDA- 2013/11/06 06:00
PMCR- 2014/03/01
CRDT- 2013/07/27 06:00
PHST- 2013/07/27 06:00 [entrez]
PHST- 2013/07/28 06:00 [pubmed]
PHST- 2013/11/06 06:00 [medline]
PHST- 2014/03/01 00:00 [pmc-release]
AID - iovs.13-12419 [pii]
AID - 10.1167/iovs.13-12419 [doi]
PST - epublish
SO  - Invest Ophthalmol Vis Sci. 2013 Sep 5;54(9):5981-8. doi: 10.1167/iovs.13-12419.