PMID- 23891138
OWN - NLM
STAT- MEDLINE
DCOM- 20140619
LR  - 20220129
IS  - 1532-2971 (Electronic)
IS  - 1090-0233 (Linking)
VI  - 198
IP  - 1
DP  - 2013 Oct
TI  - Evaluation of an IgE ELISA with Culicoides spp. extracts and recombinant salivary 
      antigens for diagnosis of insect bite hypersensitivity in Warmblood horses.
PG  - 141-7
LID - S1090-0233(13)00294-3 [pii]
LID - 10.1016/j.tvjl.2013.06.010 [doi]
AB  - Insect bite hypersensitivity (IBH) in horses represents an immunoglobulin E 
      (IgE)-mediated hypersensitivity to salivary antigens from biting midges 
      (Culicoides spp.). The aim of this study was to evaluate and compare the 
      performances of IgE ELISAs using recombinant Culicoides spp. Obsoletus group 
      salivary gland antigens or crude whole body extracts ('ObsWBE'), C. nubeculosus 
      recombinant proteins (Culn1, 3, 4, 5, 7, 8 and 10) and Obsoletus group 
      recombinant proteins (Culo1 and 2). IgE levels were measured in plasma of 343 
      Warmblood horses classified as IBH-affected (n=167) and IBH-unaffected (n=176) 
      according to the owners' descriptions. IBH-affected horses were subdivided based 
      on the severity of their clinical signs at sampling and whether or not their IBH 
      history was considered to be classical. The accuracies of the tests increased 
      when clinical signs at sampling were more pronounced or when the IBH history 
      could be considered as classical. A combination of IgE levels against the three 
      best performing Culicoides spp. recombinant proteins (Culn4, Culo1 and Culo2) and 
      ObsWBE resulted in the best performing test. When IBH-affected horses showing a 
      classical history of the disease and severe clinical signs were compared with 
      IBH-unaffected horses, the Youden's index at the optimal cut-off for the three 
      tests in combination was 0.67. This optimal cut-off had a sensitivity of 70%, a 
      specificity of 97% and a total accuracy of 92%. The performance of the IgE ELISA 
      was affected by the severity of IBH clinical signs at sampling and was improved 
      when IgE levels against several recombinant proteins were combined.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Peeters, L M
AU  - Peeters LM
AD  - Department of Biosystems, Katholieke Universiteit Leuven, BE-3001 Heverlee, 
      Belgium. Electronic address: liesbet.peeters01@gmail.com.
FAU - Janssens, S
AU  - Janssens S
FAU - Goddeeris, B M
AU  - Goddeeris BM
FAU - De Keyser, K
AU  - De Keyser K
FAU - Wilson, A D
AU  - Wilson AD
FAU - Kaufmann, C
AU  - Kaufmann C
FAU - Schaffartzik, A
AU  - Schaffartzik A
FAU - Marti, E
AU  - Marti E
FAU - Buys, N
AU  - Buys N
LA  - eng
SI  - GENBANK/JX512273
SI  - GENBANK/JX512274
GR  - BBS/E/I/00001445/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
GR  - BBS/E/I/00001701/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130725
PL  - England
TA  - Vet J
JT  - Veterinary journal (London, England : 1997)
JID - 9706281
RN  - 0 (Allergens)
RN  - 0 (Insect Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/genetics/*immunology
MH  - Animals
MH  - Ceratopogonidae/*physiology
MH  - Enzyme-Linked Immunosorbent Assay/*methods/veterinary
MH  - Horse Diseases/*diagnosis/immunology
MH  - Horses
MH  - Hypersensitivity/diagnosis/immunology/*veterinary
MH  - Immunoglobulin E/blood
MH  - Insect Bites and Stings/diagnosis/immunology/*veterinary
MH  - Insect Proteins/genetics/*immunology
MH  - Molecular Sequence Data
MH  - Recombinant Proteins/genetics/immunology
MH  - Salivary Glands/metabolism
MH  - Sequence Analysis, DNA/veterinary
OTO - NOTNLM
OT  - Allergen-specific IgE ELISA
OT  - Culicoides spp.
OT  - Equine
OT  - Insect bite hypersensitivity
OT  - Warmblood
EDAT- 2013/07/31 06:00
MHDA- 2014/06/20 06:00
CRDT- 2013/07/30 06:00
PHST- 2012/08/03 00:00 [received]
PHST- 2013/06/10 00:00 [revised]
PHST- 2013/06/13 00:00 [accepted]
PHST- 2013/07/30 06:00 [entrez]
PHST- 2013/07/31 06:00 [pubmed]
PHST- 2014/06/20 06:00 [medline]
AID - S1090-0233(13)00294-3 [pii]
AID - 10.1016/j.tvjl.2013.06.010 [doi]
PST - ppublish
SO  - Vet J. 2013 Oct;198(1):141-7. doi: 10.1016/j.tvjl.2013.06.010. Epub 2013 Jul 25.