PMID- 23891522
OWN - NLM
STAT- MEDLINE
DCOM- 20131106
LR  - 20211021
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 444
IP  - 1-2
DP  - 2013 Sep
TI  - A new nidovirus (NamDinh virus NDiV): Its ultrastructural characterization in the 
      C6/36 mosquito cell line.
PG  - 337-42
LID - S0042-6822(13)00400-5 [pii]
LID - 10.1016/j.virol.2013.06.030 [doi]
AB  - We describe the ultrastructure of the NamDinh virus (NDiV), a new member of the 
      order Nidovirales grown in the C6/36 mosquito cell line. Uninfected and 
      NDiV-infected cells were investigated by electron microscopy 24-48 h after 
      infection. The results show that the viral nucleocapsid-like particles form 
      clusters concentrated in the vacuoles, the endoplasmic reticulum, and are 
      scattered in the cytoplasm. Mature virions of NDiV were released as budding 
      particles on the cell surface where viral components appear to lie beneath and 
      along the plasma membrane. Free homogeneous virus particles were obtained by 
      ultracentrifugation on sucrose gradients of culture fluids. The size of the 
      round-shaped particles with a complete internal structure was 80 nm in diameter. 
      This is the first study to provide information on the morphogenesis and 
      ultrastructure of the first insect nidovirus NDiV, a missing evolutionary link in 
      the emergence of the viruses with the largest RNA genomes.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Thuy, Nguyen Thanh
AU  - Thuy NT
AD  - National Institute of Hygiene and Epidemiology, 1 Yersin Street, Hai Ba Trung 
      District, Hanoi, Viet Nam. ngtthuy02@yahoo.com
FAU - Huy, Tran Quang
AU  - Huy TQ
FAU - Nga, Phan Thi
AU  - Nga PT
FAU - Morita, Kouichi
AU  - Morita K
FAU - Dunia, Irene
AU  - Dunia I
FAU - Benedetti, Lucio
AU  - Benedetti L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130725
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Membrane/virology
MH  - Culicidae
MH  - Cytoplasm/virology
MH  - Microscopy, Electron
MH  - Nidovirales/*isolation & purification/physiology/*ultrastructure
MH  - Organelles/ultrastructure/virology
MH  - Virion/ultrastructure
MH  - Virus Release
PMC - PMC7111958
OTO - NOTNLM
OT  - Electron microscopy
OT  - Immunogold
OT  - NDiV
OT  - NamDinh virus
OT  - Nidovirus
OT  - Ultrastructure
EDAT- 2013/07/31 06:00
MHDA- 2013/11/07 06:00
PMCR- 2013/07/25
CRDT- 2013/07/30 06:00
PHST- 2013/04/23 00:00 [received]
PHST- 2013/05/06 00:00 [revised]
PHST- 2013/06/27 00:00 [accepted]
PHST- 2013/07/30 06:00 [entrez]
PHST- 2013/07/31 06:00 [pubmed]
PHST- 2013/11/07 06:00 [medline]
PHST- 2013/07/25 00:00 [pmc-release]
AID - S0042-6822(13)00400-5 [pii]
AID - 10.1016/j.virol.2013.06.030 [doi]
PST - ppublish
SO  - Virology. 2013 Sep;444(1-2):337-42. doi: 10.1016/j.virol.2013.06.030. Epub 2013 
      Jul 25.