PMID- 23891577
OWN - NLM
STAT- MEDLINE
DCOM- 20140529
LR  - 20191210
IS  - 1879-3177 (Electronic)
IS  - 0887-2333 (Linking)
VI  - 27
IP  - 7
DP  - 2013 Oct
TI  - Suitability of invertebrate and vertebrate cells in a portable impedance-based 
      toxicity sensor: temperature mediated impacts on long-term survival.
PG  - 2061-6
LID - S0887-2333(13)00185-9 [pii]
LID - 10.1016/j.tiv.2013.07.007 [doi]
AB  - Using ECIS (electric cell-substrate impedance sensing) to monitor the impedance 
      of vertebrate cell monolayers provides a sensitive measure of toxicity for a wide 
      range of chemical toxicants. One major limitation to using a cell-based sensor 
      for chemical toxicant detection in the field is the difficulty in maintaining 
      cell viability over extended periods of time prior to use. This research was 
      performed to identify cell lines suitable for ECIS-based toxicity sensing under 
      field conditions. A variety of invertebrate and vertebrate cell lines were 
      screened for their abilities to be stored for extended periods of time on an 
      enclosed fluidic biochip with minimal maintenance. Three of the ten cell lines 
      screened exhibited favorable portability characteristics on the biochips. 
      Interestingly, all three cell lines were derived from ectothermic vertebrates, 
      and the storage temperature that allowed long-term cell survival on the enclosed 
      fluidic biochips was also at the lower end of reported body temperature for the 
      organism, suggesting that reduced cellular metabolism may be essential for 
      longterm survival on the biochip. Future work with the ectothermic vertebrate 
      cells will characterize their sensitivity to a wide range of chemical toxicants 
      to determine if they are good candidates for use in a field portable toxicity 
      sensor.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Curtis, T M
AU  - Curtis TM
AD  - Department of Biological Sciences, State University of New York at Cortland, 
      Cortland, NY 13045-1265, United States. Electronic address: 
      Theresa.curtis@cortland.edu.
FAU - Collins, A M
AU  - Collins AM
FAU - Gerlach, B D
AU  - Gerlach BD
FAU - Brennan, L M
AU  - Brennan LM
FAU - Widder, M W
AU  - Widder MW
FAU - van der Schalie, W H
AU  - van der Schalie WH
FAU - Vo, N T K
AU  - Vo NT
FAU - Bols, N C
AU  - Bols NC
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20130725
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
RN  - 0 (Water Pollutants, Chemical)
SB  - IM
MH  - Animals
MH  - *Biosensing Techniques
MH  - Cell Culture Techniques
MH  - Cell Line
MH  - Cell Survival
MH  - Ecotoxicology/instrumentation/*methods
MH  - Electric Impedance
MH  - Environmental Monitoring/instrumentation/*methods
MH  - Epithelial Cells/*physiology
MH  - Fishes
MH  - Insecta
MH  - Lizards
MH  - Mice
MH  - Micro-Electrical-Mechanical Systems
MH  - Microfluidics/methods
MH  - Rana pipiens
MH  - Reproducibility of Results
MH  - Species Specificity
MH  - Temperature
MH  - Water Pollutants, Chemical/*toxicity
OTO - NOTNLM
OT  - Biochip
OT  - Biosensor
OT  - Cell monolayer storage
OT  - ECIS
OT  - Ectotherms
OT  - Electric cell-substrate impedance sensing
EDAT- 2013/07/31 06:00
MHDA- 2014/05/30 06:00
CRDT- 2013/07/30 06:00
PHST- 2013/03/12 00:00 [received]
PHST- 2013/06/25 00:00 [revised]
PHST- 2013/07/15 00:00 [accepted]
PHST- 2013/07/30 06:00 [entrez]
PHST- 2013/07/31 06:00 [pubmed]
PHST- 2014/05/30 06:00 [medline]
AID - S0887-2333(13)00185-9 [pii]
AID - 10.1016/j.tiv.2013.07.007 [doi]
PST - ppublish
SO  - Toxicol In Vitro. 2013 Oct;27(7):2061-6. doi: 10.1016/j.tiv.2013.07.007. Epub 
      2013 Jul 25.