PMID- 23891890
OWN - NLM
STAT- MEDLINE
DCOM- 20140401
LR  - 20130827
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 149
IP  - 2
DP  - 2013 Sep 16
TI  - Effects of magnolialide isolated from the leaves of Laurus nobilis L. (Lauraceae) 
      on immunoglobulin E-mediated type I hypersensitivity in vitro.
PG  - 550-6
LID - S0378-8741(13)00504-7 [pii]
LID - 10.1016/j.jep.2013.07.015 [doi]
AB  - ETHNOPHARMACOLOGICAL RELEVANCE: Laurus nobilis L. (Lauraceae) has been used for 
      folk medicines in the Mediterranean area and Europe to treat various disorders 
      including skin inflammation (dermatitis) and asthma. AIM OF THE STUDY: Our aim 
      was to investigate the scientific evaluation of the compounds from Laurus nobilis 
      L. on immuniglobulin E (IgE)-mediated type I hypersensitivity responses in vitro 
      such as atopic dermatitis and asthma. METHODS AND MATERIALS: Seven compounds were 
      isolated and examined for the mast cell stabilizing effect on IgE-sensitized 
      RBL-2H3 mast cells by measuring the beta-hexosaminidase activity. In addition, the 
      effects on interleukin (IL)-4 production and IL-5-dependent Y16 early B cell 
      proliferation were investigated as well as their cytotoxic effects on RBL-2H3 
      cells. RESULTS: Among the seven isolated compounds, magnolialide attenuated the 
      release of beta-hexosaminidase from RBL-2H3 cells with an IC50 value of 20.2 muM, 
      while the other compounds revealed no significant effects at concentrations 
      tested. Furthermore, magnolialide significantly inhibited the IL-4 release with 
      an IC50 value of 18.1 muM and IL-4 mRNA expression with an IC50 value of 15.7 muM 
      in IgE-sensitized RBL-2H3 cells. In addition, the inhibition of IL-5-dependent 
      proliferation of early B cells (Y16 cells) by magnolialide was demonstrated with 
      an IC50 value of 18.4 muM. CONCLUSION: These results suggest that the magnolialide 
      might be a candidate for the treatment of IgE-mediated hypersensitivity responses 
      such as atopic dermatitis and asthma by inhibiting mast cell degranulation, the 
      IL-4 production, and IL-5-dependent early B cell proliferation, key factors in 
      the development and amplification of type I hypersensitivity reactions.
CI  - (c) 2013 Elsevier Ireland Ltd. All rights reserved.
FAU - Lee, Taehun
AU  - Lee T
AD  - Natural Products Research Institute, College of Pharmacy, Seoul National 
      University, Gwanak-gu, Seoul, Republic of Korea.
FAU - Lee, Sooryun
AU  - Lee S
FAU - Ho Kim, Kyeong
AU  - Ho Kim K
FAU - Oh, Ki-Bong
AU  - Oh KB
FAU - Shin, Jongheon
AU  - Shin J
FAU - Mar, Woongchon
AU  - Mar W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130724
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Anti-Allergic Agents)
RN  - 0 (Dinitrophenols)
RN  - 0 (Haptens)
RN  - 0 (Lactones)
RN  - 0 (RNA, Messenger)
RN  - 0 (Serum Albumin)
RN  - 0 (Sesquiterpenes)
RN  - 0 (dinitrophenyl-human serum albumin conjugate)
RN  - 0 (magnolialide)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.2.1.52 (beta-N-Acetylhexosaminidases)
SB  - IM
MH  - Animals
MH  - Anti-Allergic Agents/*pharmacology
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Dinitrophenols/immunology
MH  - Haptens/immunology
MH  - Hypersensitivity/immunology
MH  - Immunoglobulin E/immunology
MH  - Interleukin-4/genetics/metabolism
MH  - Lactones/*pharmacology
MH  - *Laurus
MH  - Mice
MH  - Plant Leaves
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Serum Albumin/immunology
MH  - Sesquiterpenes/*pharmacology
MH  - beta-N-Acetylhexosaminidases/metabolism
OTO - NOTNLM
OT  - Interleukin-4
OT  - Interleukin-5
OT  - Lauraceae
OT  - Laurus nobilis
OT  - Magnolialide
OT  - beta-hexosaminidase
EDAT- 2013/07/31 06:00
MHDA- 2014/04/02 06:00
CRDT- 2013/07/30 06:00
PHST- 2013/03/25 00:00 [received]
PHST- 2013/07/04 00:00 [revised]
PHST- 2013/07/11 00:00 [accepted]
PHST- 2013/07/30 06:00 [entrez]
PHST- 2013/07/31 06:00 [pubmed]
PHST- 2014/04/02 06:00 [medline]
AID - S0378-8741(13)00504-7 [pii]
AID - 10.1016/j.jep.2013.07.015 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2013 Sep 16;149(2):550-6. doi: 10.1016/j.jep.2013.07.015. Epub 
      2013 Jul 24.