PMID- 23891891
OWN - NLM
STAT- MEDLINE
DCOM- 20140826
LR  - 20131211
IS  - 1873-2933 (Electronic)
IS  - 0009-9120 (Linking)
VI  - 46
IP  - 18
DP  - 2013 Dec
TI  - Normative levels of Interleukin 16 in umbilical cord blood.
PG  - 1857-9
LID - S0009-9120(13)00345-7 [pii]
LID - 10.1016/j.clinbiochem.2013.07.012 [doi]
AB  - OBJECTIVES: The need for early and accurate prediction of outcome in 
      hypoxic-ischaemic encephalopathy (HIE) remains critical. We have previously 
      demonstrated that Interleukin 16 (IL-16) is raised in the umbilical cord blood 
      (UCB) of infants with moderate and severe HIE and has the potential to be 
      developed as a predictive biomarker. Normal reference ranges for IL-16 in UCB 
      have not been previously described. The aim of this study was to determine 
      normative levels of IL-16 in full term neonates using UCB following uncomplicated 
      deliveries and to examine the effect of labour on cord IL-16 values. DESIGN AND 
      METHODS: Full term infants were recruited as part of an ongoing birth cohort 
      study, the Cork BASELINE Birth Cohort Study. All had UCB drawn and bio-banked at 
      -80 degrees C, within 3hours of birth. Samples for this experiment were chosen from this 
      population based cohort study to represent uncomplicated pre-labour caesarean 
      sections and spontaneous vaginal deliveries. Analysis was performed on plasma 
      EDTA, using ELISA Quantikine(R) (R&D Systems, Europe). RESULTS: Samples were 
      analysed from 48 infants with two modes of delivery; spontaneous vaginal delivery 
      (n=12 male, n=12 female) and elective caesarean section (n=12 male, n=12 female). 
      The range of all samples was normally distributed between 87.0 and 114.6pg/ml. 
      Overall mean (SD) for IL-16 was 102.9 (21.5) pg/ml. Levels were not affected by 
      spontaneous vaginal delivery or gender. CONCLUSION: For the first time we have 
      described the expected range of cord plasma IL-16 levels in healthy term infants 
      following pre-labour and post-labour delivery.
CI  - (c) 2013.
FAU - Denihan, N M
AU  - Denihan NM
AD  - Neonatal Brain Research Group, Department of Paediatrics and Child Health, Cork 
      University Maternity Hospital, Wilton, Cork, Ireland. Electronic address: 
      n.denihan@ucc.ie.
FAU - Looney, Am
AU  - Looney A
FAU - Boylan, G B
AU  - Boylan GB
FAU - Walsh, B H
AU  - Walsh BH
FAU - Murray, D M
AU  - Murray DM
LA  - eng
SI  - ClinicalTrials.gov/NCT01498965
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130724
PL  - United States
TA  - Clin Biochem
JT  - Clinical biochemistry
JID - 0133660
RN  - 0 (Interleukin-16)
SB  - IM
MH  - Cesarean Section
MH  - Cohort Studies
MH  - Female
MH  - Fetal Blood/*chemistry
MH  - Humans
MH  - Infant, Newborn
MH  - Interleukin-16/*blood
MH  - Labor, Obstetric/blood
MH  - Male
MH  - Pregnancy
MH  - *Reference Values
OTO - NOTNLM
OT  - Cytokines
OT  - ELISA
OT  - Elective caesarean section
OT  - Hypoxic-ischaemic encephalopathy
OT  - Interleukin 16
OT  - Pregnancy
OT  - Umbilical cord blood
OT  - Vaginal delivery
EDAT- 2013/07/31 06:00
MHDA- 2014/08/27 06:00
CRDT- 2013/07/30 06:00
PHST- 2013/04/11 00:00 [received]
PHST- 2013/07/02 00:00 [revised]
PHST- 2013/07/13 00:00 [accepted]
PHST- 2013/07/30 06:00 [entrez]
PHST- 2013/07/31 06:00 [pubmed]
PHST- 2014/08/27 06:00 [medline]
AID - S0009-9120(13)00345-7 [pii]
AID - 10.1016/j.clinbiochem.2013.07.012 [doi]
PST - ppublish
SO  - Clin Biochem. 2013 Dec;46(18):1857-9. doi: 10.1016/j.clinbiochem.2013.07.012. 
      Epub 2013 Jul 24.