PMID- 23891913
OWN - NLM
STAT- MEDLINE
DCOM- 20140218
LR  - 20191210
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 934
DP  - 2013 Sep 1
TI  - Ultra-performance liquid chromatography-tandem mass spectrometry determination 
      and depletion profile of flunixin residues in tissues after single oral 
      administration in rabbits.
PG  - 8-15
LID - S1570-0232(13)00360-7 [pii]
LID - 10.1016/j.jchromb.2013.07.002 [doi]
AB  - An ultra-performance liquid chromatography with tandem mass spectrometric 
      detection (UPLC-MS/MS) method was developed for the detection of flunixin 
      residues in rabbit tissues. The samples were extracted with acidic acetonitrile, 
      defatted with n-hexane, and then purified by HLB solid-phase extraction 
      cartridge. Analysis was carried out on UPLC-ESI-MS/MS working with multiple 
      reaction monitoring (MRM) mode. The limits of detection (LODs) of the method were 
      0.3-0.8mugkg(-1) and limits of quantification (LOQs) were 1.0-3.0mugkg(-1) in 
      rabbit tissues, respectively. In all fortified samples at a concentration range 
      of 1.0-300.0mugkg(-1), mean recoveries were 61.7-115.7% with relative standard 
      deviations (RSDs) below 16%. Residue depletion of flunixin in rabbit was 
      conducted after oral administration at a dose of 5mgkg(-1) of body weight. The 
      average concentrations for flunixin measured 2h post-administration in kidney and 
      intestine were significantly higher than in liver, heart and muscle. The 
      concentrations for flunixin in all rabbit tissues were below the LOD or not 
      detected in all tissues after 96h administration of drug. A minimum withdrawal 
      time of 21h was indicated for residue levels in heart, liver, kidney, intestine 
      and muscle below the maximum residue limits (MRLs).
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Zhu, Ai-Ling
AU  - Zhu AL
AD  - College of Veterinary Medicine of China Agricultural University, Beijing 100193, 
      PR China.
FAU - Peng, Tao
AU  - Peng T
FAU - Liu, Liang
AU  - Liu L
FAU - Xia, Xi
AU  - Xia X
FAU - Hu, Ting
AU  - Hu T
FAU - Tao, Xiao-Qi
AU  - Tao XQ
FAU - Wen, Kai
AU  - Wen K
FAU - Cheng, Lin-Li
AU  - Cheng LL
FAU - Li, Jian-Cheng
AU  - Li JC
FAU - Ding, Shuang-Yang
AU  - Ding SY
FAU - Cao, Xing-Yuan
AU  - Cao XY
FAU - Jiang, Hai-Yang
AU  - Jiang HY
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130708
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 356IB1O400 (flunixin)
RN  - V7DXN0M42R (Clonixin)
SB  - IM
MH  - Animals
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Clonixin/administration & dosage/*analogs & derivatives/analysis/pharmacokinetics
MH  - Drug Monitoring/*methods
MH  - Drug Residues/analysis/pharmacokinetics
MH  - Intestines/chemistry
MH  - Kidney/chemistry
MH  - Liver/chemistry
MH  - Muscles/chemistry
MH  - Rabbits
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - Flunixin
OT  - Rabbit tissue
OT  - Residue depletion
OT  - Ultra-performance liquid chromatography tandem mass spectrometry
OT  - Withdrawal time
EDAT- 2013/07/31 06:00
MHDA- 2014/02/19 06:00
CRDT- 2013/07/30 06:00
PHST- 2013/05/02 00:00 [received]
PHST- 2013/06/29 00:00 [revised]
PHST- 2013/07/01 00:00 [accepted]
PHST- 2013/07/30 06:00 [entrez]
PHST- 2013/07/31 06:00 [pubmed]
PHST- 2014/02/19 06:00 [medline]
AID - S1570-0232(13)00360-7 [pii]
AID - 10.1016/j.jchromb.2013.07.002 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Sep 1;934:8-15. doi: 
      10.1016/j.jchromb.2013.07.002. Epub 2013 Jul 8.