PMID- 23892595
OWN - NLM
STAT- MEDLINE
DCOM- 20140407
LR  - 20211021
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 14
IP  - 8
DP  - 2013 Jul 25
TI  - Apoptosis signal-regulating kinase 1 is involved in brain-derived neurotrophic 
      factor (BDNF)-enhanced cell motility and matrix metalloproteinase 1 expression in 
      human chondrosarcoma cells.
PG  - 15459-78
LID - 10.3390/ijms140815459 [doi]
AB  - Chondrosarcoma is the primary malignancy of bone that is characterized by a 
      potent capacity to invade locally and cause distant metastasis, and is therefore 
      associated with poor prognoses. Chondrosarcoma further shows a predilection for 
      metastasis to the lungs. The brain-derived neurotrophic factor (BDNF) is a small 
      molecule in the neurotrophin family of growth factors that is associated with the 
      disease status and outcome of cancers. However, the effect of BDNF on cell 
      motility in human chondrosarcoma cells is mostly unknown. Here, we found that 
      human chondrosarcoma cell lines had significantly higher cell motility and BDNF 
      expression compared to normal chondrocytes. We also found that BDNF increased 
      cell motility and expression of matrix metalloproteinase-1 (MMP-1) in human 
      chondrosarcoma cells. BDNF-mediated cell motility and MMP-1 up-regulation were 
      attenuated by Trk inhibitor (K252a), ASK1 inhibitor (thioredoxin), JNK inhibitor 
      (SP600125), and p38 inhibitor (SB203580). Furthermore, BDNF also promoted Sp1 
      activation. Our results indicate that BDNF enhances the migration and invasion 
      activity of chondrosarcoma cells by increasing MMP-1 expression through a signal 
      transduction pathway that involves the TrkB receptor, ASK1, JNK/p38, and Sp1. 
      BDNF thus represents a promising new target for treating chondrosarcoma 
      metastasis.
FAU - Lin, Chih-Yang
AU  - Lin CY
AD  - Graduate Institute of Basic Medical Science, China Medical University, Taichung 
      404, Taiwan. p123400@hotmail.com
FAU - Chang, Sunny Li-Yun
AU  - Chang SL
FAU - Fong, Yi-Chin
AU  - Fong YC
FAU - Hsu, Chin-Jung
AU  - Hsu CJ
FAU - Tang, Chih-Hsin
AU  - Tang CH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130725
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Anthracenes)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Carbazoles)
RN  - 0 (Imidazoles)
RN  - 0 (Indole Alkaloids)
RN  - 0 (Pyridines)
RN  - 1TW30Y2766 (pyrazolanthrone)
RN  - 52500-60-4 (Thioredoxins)
RN  - 7171WSG8A2 (BDNF protein, human)
RN  - 97161-97-2 (staurosporine aglycone)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.- (Sp1 kinase)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.25 (MAP Kinase Kinase Kinase 5)
RN  - EC 2.7.11.25 (MAP3K5 protein, human)
RN  - EC 3.4.24.7 (MMP1 protein, human)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
RN  - OU13V1EYWQ (SB 203580)
SB  - IM
MH  - Anthracenes/pharmacology
MH  - Bone Neoplasms/*metabolism/mortality
MH  - Brain-Derived Neurotrophic Factor/biosynthesis/*metabolism
MH  - Carbazoles/pharmacology
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Chondrosarcoma/*metabolism/mortality
MH  - Humans
MH  - Imidazoles/pharmacology
MH  - Indole Alkaloids/pharmacology
MH  - JNK Mitogen-Activated Protein Kinases/antagonists & inhibitors
MH  - MAP Kinase Kinase Kinase 5/antagonists & inhibitors/*metabolism
MH  - Matrix Metalloproteinase 1/biosynthesis/*metabolism
MH  - Neoplasm Invasiveness
MH  - Neoplasm Metastasis
MH  - Prognosis
MH  - Protein Kinases/metabolism
MH  - Pyridines/pharmacology
MH  - Receptor, trkB/antagonists & inhibitors
MH  - Signal Transduction
MH  - Thioredoxins/pharmacology
MH  - Up-Regulation
MH  - p38 Mitogen-Activated Protein Kinases/antagonists & inhibitors
PMC - PMC3759868
EDAT- 2013/07/31 06:00
MHDA- 2014/04/08 06:00
PMCR- 2013/08/01
CRDT- 2013/07/30 06:00
PHST- 2013/05/21 00:00 [received]
PHST- 2013/07/15 00:00 [revised]
PHST- 2013/07/15 00:00 [accepted]
PHST- 2013/07/30 06:00 [entrez]
PHST- 2013/07/31 06:00 [pubmed]
PHST- 2014/04/08 06:00 [medline]
PHST- 2013/08/01 00:00 [pmc-release]
AID - ijms140815459 [pii]
AID - ijms-14-15459 [pii]
AID - 10.3390/ijms140815459 [doi]
PST - epublish
SO  - Int J Mol Sci. 2013 Jul 25;14(8):15459-78. doi: 10.3390/ijms140815459.