PMID- 23894566
OWN - NLM
STAT- MEDLINE
DCOM- 20140327
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 7
DP  - 2013
TI  - Drosophila Hephaestus/polypyrimidine tract binding protein is required for 
      dorso-ventral patterning and regulation of signalling between the germline and 
      soma.
PG  - e69978
LID - 10.1371/journal.pone.0069978 [doi]
LID - e69978
AB  - In the Drosophila oocyte, gurken (grk) mRNA encodes a secreted TGF-alpha signal that 
      specifies the future embryonic dorso-ventral axes by altering the fate of the 
      surrounding epithelial follicle cells. We previously identified a number of RNA 
      binding proteins that associate specifically with the 64 nucleotide grk 
      localization signal, including the Drosophila orthologue of polypyrimidine 
      tract-binding protein (PTB), Hephaestus (Heph). To test whether Heph is required 
      for correct grk mRNA or protein function, we used immunoprecipitation to validate 
      the association of Heph with grk mRNA and characterized the heph mutant 
      phenotype. We found that Heph is a component of grk mRNP complexes but heph 
      germline clones show that Heph is not required for grk mRNA localization. 
      Instead, we identify a novel function for Heph in the germline and show that it 
      is required for proper Grk protein localization. Furthermore, we show that Heph 
      is required in the oocyte for the correct organization of the actin cytoskeleton 
      and dorsal appendage morphogenesis. Our results highlight a requirement for an 
      mRNA binding protein in the localization of Grk protein, which is independent of 
      mRNA localization, and we propose that Heph is required in the germline for 
      efficient Grk signalling to the somatic follicle cells during dorso-ventral 
      patterning.
FAU - McDermott, Suzanne M
AU  - McDermott SM
AD  - Department of Biochemistry, University of Oxford, Oxford, United Kingdom. 
      suzanne.mcdermott@seattlebiomed.org
FAU - Davis, Ilan
AU  - Davis I
LA  - eng
GR  - Wellcome Trust/United Kingdom
GR  - 096144/Wellcome Trust/United Kingdom
GR  - 081858/WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130723
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Drosophila Proteins)
RN  - 0 (Heph protein, Drosophila)
RN  - 0 (RNA, Messenger)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (Transforming Growth Factor alpha)
RN  - 0 (grk protein, Drosophila)
RN  - 139076-35-0 (Polypyrimidine Tract-Binding Protein)
SB  - IM
MH  - Actin Cytoskeleton/metabolism
MH  - Animals
MH  - Body Patterning
MH  - Drosophila Proteins/genetics/*metabolism
MH  - Drosophila melanogaster/embryology/growth & development/*metabolism
MH  - Female
MH  - Germ Cells/*metabolism
MH  - Male
MH  - Mutation
MH  - Oocytes/metabolism
MH  - Polypyrimidine Tract-Binding Protein/genetics/*metabolism
MH  - RNA Transport
MH  - RNA, Messenger/genetics/metabolism
MH  - Ribonucleoproteins/metabolism
MH  - Signal Transduction
MH  - Transforming Growth Factor alpha/genetics/metabolism
PMC - PMC3720928
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/07/31 06:00
MHDA- 2014/03/29 06:00
PMCR- 2013/07/23
CRDT- 2013/07/30 06:00
PHST- 2013/02/08 00:00 [received]
PHST- 2013/06/14 00:00 [accepted]
PHST- 2013/07/30 06:00 [entrez]
PHST- 2013/07/31 06:00 [pubmed]
PHST- 2014/03/29 06:00 [medline]
PHST- 2013/07/23 00:00 [pmc-release]
AID - PONE-D-13-05732 [pii]
AID - 10.1371/journal.pone.0069978 [doi]
PST - epublish
SO  - PLoS One. 2013 Jul 23;8(7):e69978. doi: 10.1371/journal.pone.0069978. Print 2013.