PMID- 23895426
OWN - NLM
STAT- MEDLINE
DCOM- 20140403
LR  - 20130912
IS  - 1365-2826 (Electronic)
IS  - 0953-8194 (Linking)
VI  - 25
IP  - 10
DP  - 2013 Oct
TI  - Endocannabinoid receptor deficiency affects maternal care and alters the dam's 
      hippocampal oxytocin receptor and brain-derived neurotrophic factor expression.
PG  - 898-909
LID - 10.1111/jne.12082 [doi]
AB  - Maternal care is the newborn's first experience of social interaction, and this 
      influences infant survival, development and social competences throughout life. 
      We recently found that postpartum blocking of the endocannabinoid receptor-1 
      (CB1R) altered maternal behaviour. In the present study, maternal care was 
      assessed by the time taken to retrieve pups, pups' ultrasonic vocalisations 
      (USVs) and pup body weight, comparing CB1R deleted (CB1R KO) versus wild-type 
      (WT) mice. After culling on postpartum day 8, hippocampal expression of oxytocin 
      receptor (OXTR), brain-derived neurotrophic factor (BDNF) and stress-mediating 
      factors were evaluated in CB1R KO and WT dams. Comparisons were also performed 
      with nulliparous (NP) CB1R KO and WT mice. Compared to WT, CB1R KO dams were 
      slower to retrieve their pups. Although the body weight of the KO pups did not 
      differ from the weight of WT pups, they emitted fewer USVs. This impairment of 
      the dam-pup relationship correlated with a significant reduction of OXTR mRNA and 
      protein levels among CB1R KO dams compared to WT dams. Furthermore, WT dams 
      exhibited elevated OXTR mRNA expression, as well as increased levels of 
      mineralocorticoid and glucocorticoid receptors, compared to WT NP mice. By 
      contrast, CB1R KO dams showed no such elevation of OXTR expression, alongside 
      lower BDNF and mineralocorticoid receptors, as well as elevated 
      corticotrophin-releasing hormone mRNA levels, when compared to CB1R KO NP. Thus, 
      it appears that the disruption of endocannabinoid signalling by CB1R deletion 
      alters expression of the OXTR, apparently leading to deleterious effects upon 
      maternal behaviour.
CI  - (c) 2013 British Society for Neuroendocrinology.
FAU - Schechter, M
AU  - Schechter M
AD  - Department of Molecular Biology, Ariel University, Ariel, Israel; Faculty of Life 
      Sciences, Bar Ilan University, Ramat-Gan, Israel; Gonda Brain Research Center, 
      Bar Ilan University, Ramat-Gan, Israel.
FAU - Weller, A
AU  - Weller A
FAU - Pittel, Z
AU  - Pittel Z
FAU - Gross, M
AU  - Gross M
FAU - Zimmer, A
AU  - Zimmer A
FAU - Pinhasov, A
AU  - Pinhasov A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neuroendocrinol
JT  - Journal of neuroendocrinology
JID - 8913461
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cnr1 protein, rat)
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptor, Cannabinoid, CB1)
RN  - 0 (Receptors, Cannabinoid)
RN  - 0 (Receptors, Oxytocin)
RN  - 50-56-6 (Oxytocin)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - *Behavior, Animal
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - DNA Primers
MH  - Female
MH  - Hippocampus/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Oxytocin/blood
MH  - RNA, Messenger/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Receptor, Cannabinoid, CB1/*metabolism
MH  - Receptors, Cannabinoid/*metabolism
MH  - Receptors, Oxytocin/genetics/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
OTO - NOTNLM
OT  - CB1 receptor
OT  - brain-derived neurotrophic factor
OT  - corticosterone
OT  - endocannabinoid system
OT  - maternal care
OT  - oxytocin receptor
EDAT- 2013/07/31 06:00
MHDA- 2014/04/04 06:00
CRDT- 2013/07/31 06:00
PHST- 2012/06/29 00:00 [received]
PHST- 2013/07/10 00:00 [revised]
PHST- 2013/07/24 00:00 [accepted]
PHST- 2013/07/31 06:00 [entrez]
PHST- 2013/07/31 06:00 [pubmed]
PHST- 2014/04/04 06:00 [medline]
AID - 10.1111/jne.12082 [doi]
PST - ppublish
SO  - J Neuroendocrinol. 2013 Oct;25(10):898-909. doi: 10.1111/jne.12082.