PMID- 23895636
OWN - NLM
STAT- MEDLINE
DCOM- 20140331
LR  - 20200914
IS  - 1362-301X (Electronic)
IS  - 0269-9052 (Linking)
VI  - 27
IP  - 10
DP  - 2013
TI  - Effect of focal mild hypothermia on expression of MMP-9, TIMP-1, Tau-1 and beta-APP 
      in rats with cerebral ischaemia/reperfusion injury.
PG  - 1190-8
LID - 10.3109/02699052.2013.804206 [doi]
AB  - PRIMARY OBJECTIVE: Following stroke, hypothermia is reported to reduce both 
      cellular and extracellular damage. This study aimed to examine the effects of 
      focal mild hypothermia on proteins associated with both extracellular (matrix 
      metalloproteinase-9 (MMP-9) and tissue inhibitor of MMP-9 (TIMP-1)) and cellular 
      damage (Tau-1 and beta-amyloid precursor protein (beta-APP)) to characterize the 
      protective effects of hypothermia. METHODS AND PROCEDURES: Male Wistar rats 
      received ischaemic damage using a transient, focal ischaemia/reperfusion model. 
      Afterwards, one group (HT) received 6 hours of focal mild hypothermia (33  degrees C) 
      applied to the head, while another remained at normal temperature (NT). The 
      brains were collected at 6, 12, 24, 48 and 72 hours after hypothermia to measure 
      infarct volume ratio and to detect cells immunopositive for MMP-9, TIMP-1, Tau-1 
      and beta-APP, while neurological deficits were examined separately after 2 weeks. 
      MAIN OUTCOMES AND RESULTS: Focal mild hypothermia had no effect on infarct volume 
      ratio but expression of MMP-9, TIMP-1 Tau-1 and beta-APP was decreased. Furthermore, 
      neurological function in the HT group was better than in the NT group. 
      CONCLUSIONS: Focal mild hypothermia has protective effects on cerebral 
      ischaemia-reperfusion injury characterized by decreased expression of MMP-9, 
      TIMP-1, Tau-1 and beta-APP, along with improvement of neurological function despite 
      no changes in infarct volume.
FAU - Zhao, Jing-Kun
AU  - Zhao JK
AD  - Department of Neurology, The First Affiliated Hospital of Harbin Medical 
      University, Nangang District, Harbin, PR China.
FAU - Guan, Fu-Lin
AU  - Guan FL
FAU - Duan, Shu-Rong
AU  - Duan SR
FAU - Zhao, Ji-Wei
AU  - Zhao JW
FAU - Sun, Rui-Hong
AU  - Sun RH
FAU - Zhang, Li-Ming
AU  - Zhang LM
FAU - Wang, De-Sheng
AU  - Wang DS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130729
PL  - England
TA  - Brain Inj
JT  - Brain injury
JID - 8710358
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Mapt protein, rat)
RN  - 0 (Peptide Fragments)
RN  - 0 (TIMP1 protein, rat)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 0 (amyloid beta-protein precursor 770 (681-695))
RN  - 0 (tau Proteins)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - EC 3.4.24.35 (Mmp9 protein, rat)
SB  - IM
MH  - Amyloid beta-Protein Precursor/*metabolism
MH  - Analysis of Variance
MH  - Animals
MH  - Brain/*metabolism
MH  - Cerebral Infarction/*metabolism/pathology/*therapy
MH  - Down-Regulation
MH  - *Hypothermia, Induced
MH  - Immunohistochemistry
MH  - Male
MH  - Matrix Metalloproteinase 9/*metabolism
MH  - Peptide Fragments/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Recovery of Function
MH  - Reperfusion Injury/*metabolism/*therapy
MH  - Tissue Inhibitor of Metalloproteinase-1/*metabolism
MH  - tau Proteins/*metabolism
EDAT- 2013/07/31 06:00
MHDA- 2014/04/01 06:00
CRDT- 2013/07/31 06:00
PHST- 2013/07/31 06:00 [entrez]
PHST- 2013/07/31 06:00 [pubmed]
PHST- 2014/04/01 06:00 [medline]
AID - 10.3109/02699052.2013.804206 [doi]
PST - ppublish
SO  - Brain Inj. 2013;27(10):1190-8. doi: 10.3109/02699052.2013.804206. Epub 2013 Jul 
      29.