PMID- 23896347
OWN - NLM
STAT- MEDLINE
DCOM- 20140423
LR  - 20191210
IS  - 1095-8274 (Electronic)
IS  - 1075-9964 (Linking)
VI  - 23
DP  - 2013 Oct
TI  - Simultaneous visualization of Propionibacterium acnes and Propionibacterium 
      granulosum with immunofluorescence and fluorescence in situ hybridization.
PG  - 48-54
LID - S1075-9964(13)00123-6 [pii]
LID - 10.1016/j.anaerobe.2013.07.002 [doi]
AB  - Propionibacterium acnes (P. acnes) and Propionibacterium granulosum 
      (P. granulosum) are common skin colonizers that are implicated as possible 
      contributing factors in acne vulgaris development. We have established direct 
      visualization tools for the simultaneous detection of these closely related 
      species with immunofluorescence assay and fluorescence in situ hybridization 
      (FISH). As proof of principle, we were able to distinguish P. acnes and 
      P. granulosum bacteria in multi-species populations in vitro as well as in a mock 
      skin infection model upon labelling with 16S rRNA probes in combinatorial FISH as 
      well as with antibodies. Furthermore, we report the co-localization of P. acnes 
      and P. granulosum in the stratum corneum and hair follicles from patients with 
      acne vulgaris as well as in healthy individuals. Further studies on the spatial 
      distribution of these bacteria in skin structures in various skin disorders are 
      needed.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Jahns, Anika C
AU  - Jahns AC
AD  - Department of Medical Biosciences/Pathology, Umea University, Sweden.
FAU - Oprica, Cristina
AU  - Oprica C
FAU - Vassilaki, Ismini
AU  - Vassilaki I
FAU - Golovleva, Irina
AU  - Golovleva I
FAU - Palmer, Ruth H
AU  - Palmer RH
FAU - Alexeyev, Oleg A
AU  - Alexeyev OA
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130727
PL  - England
TA  - Anaerobe
JT  - Anaerobe
JID - 9505216
SB  - IM
MH  - Acne Vulgaris/*microbiology
MH  - Fluorescent Antibody Technique/*methods
MH  - Hair Follicle/microbiology
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Propionibacterium/*classification/*genetics
MH  - Skin/microbiology
OTO - NOTNLM
OT  - FISH
OT  - Propionibacterium acnes
OT  - Propionibacterium granulosum
OT  - Skin
EDAT- 2013/07/31 06:00
MHDA- 2014/04/24 06:00
CRDT- 2013/07/31 06:00
PHST- 2013/05/27 00:00 [received]
PHST- 2013/07/11 00:00 [revised]
PHST- 2013/07/16 00:00 [accepted]
PHST- 2013/07/31 06:00 [entrez]
PHST- 2013/07/31 06:00 [pubmed]
PHST- 2014/04/24 06:00 [medline]
AID - S1075-9964(13)00123-6 [pii]
AID - 10.1016/j.anaerobe.2013.07.002 [doi]
PST - ppublish
SO  - Anaerobe. 2013 Oct;23:48-54. doi: 10.1016/j.anaerobe.2013.07.002. Epub 2013 Jul 
      27.