PMID- 23896880
OWN - NLM
STAT- MEDLINE
DCOM- 20140224
LR  - 20130730
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1044
DP  - 2013
TI  - Fluorescence in situ hybridization (FISH) technique for the micronucleus test.
PG  - 237-44
LID - 10.1007/978-1-62703-529-3_12 [doi]
AB  - In recent years, cytogenetics in combination with molecular methods has made 
      rapid progress, resulting in new molecular cytogenetic methodologies such as 
      fluorescence in situ hybridization (FISH). FISH is a molecular cytogenetic 
      technique used for the detection of specific chromosomal rearrangements and 
      applicable to many different specimen types. It uses fluorescently labeled DNA 
      probes complementary to regions of individual chromosomes. These labeled DNA 
      segments hybridize with the cytological targets in the sample and can be 
      visualized by fluorescence microscopy in interphase nuclei or on metaphase 
      chromosomes. Here, we describe the FISH methodology with centromeric probes for 
      human cells, which is used in combination with the cytokinesis-block micronucleus 
      assay and which allows discrimination between mutagens inducing DNA breakage 
      (clastogens) or chromosome loss (aneugens).
FAU - Decordier, Ilse
AU  - Decordier I
AD  - Laboratorium voor Cellulaire Genetica, Vrije Universiteit Brussel, Brussels, 
      Belgium.
FAU - Kirsch-Volders, Micheline
AU  - Kirsch-Volders M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Mutagens)
SB  - IM
MH  - Chromosomes/drug effects/genetics
MH  - Genomic Instability/drug effects/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymphocytes/cytology/drug effects/metabolism
MH  - Micronucleus Tests/*methods
MH  - Mutagens/toxicity
EDAT- 2013/07/31 06:00
MHDA- 2014/02/25 06:00
CRDT- 2013/07/31 06:00
PHST- 2013/07/31 06:00 [entrez]
PHST- 2013/07/31 06:00 [pubmed]
PHST- 2014/02/25 06:00 [medline]
AID - 10.1007/978-1-62703-529-3_12 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2013;1044:237-44. doi: 10.1007/978-1-62703-529-3_12.