PMID- 23897745
OWN - NLM
STAT- MEDLINE
DCOM- 20161031
LR  - 20161230
IS  - 1932-7005 (Electronic)
IS  - 1932-6254 (Linking)
VI  - 10
IP  - 2
DP  - 2016 Feb
TI  - The essential role of annexin A1 mimetic peptide in the skin allograft survival.
PG  - E44-53
LID - 10.1002/term.1773 [doi]
AB  - Immunosuppressive drugs have a critical role in inhibiting tissue damage and 
      allograft rejection. Studies have demonstrated the anti-inflammatory effects of 
      the annexin A1 (AnxA1) in the regulation of transmigration and apoptosis of 
      leucocytes. In the present study, an experimental skin allograft model was used 
      to evaluate a potential protective effect of AnxA1 in transplantation survival. 
      Mice were used for the skin allograft model and pharmacological treatments were 
      carried out using either the AnxA1 mimetic peptide Ac2-26, with or without 
      cyclosporine A (CsA), starting 3 days before surgery until rejection. Graft 
      survival, skin histopathology, leucocyte transmigration and expression of AnxA1 
      and AnxA5 post-transplantation were analysed. Pharmacological treatment with 
      Ac2-26 increased skin allograft survival related with inhibition of neutrophil 
      transmigration and induction of apoptosis, thereby reducing the tissue damage 
      compared with control animals. Moreover, AnxA1 and AnxA5 expression increased 
      after Ac2-26 treatment in neutrophils. Interestingly, the combination of Ac2-26 
      and cyclosporine A showed similar survival of transplants when compared with the 
      cyclosporine A group, which could be attributed to a synergistic effect of both 
      drugs. Investigations in vitro revealed that cyclosporine A inhibited 
      extracellular-signal-regulated kinase (ERK) phosphorylation induced by Ac2-26 in 
      neutrophils. Overall, the results suggest that AnxA1 has an essential role in 
      augmenting the survival of skin allograft, mainly owing to inhibition of 
      neutrophil transmigration and enhancement of apoptosis. This effect may lead to 
      the development of new therapeutic approaches relevant to transplant rejection.
CI  - Copyright (c) 2013 John Wiley & Sons, Ltd.
FAU - Teixeira, Rodrigo Antonio Parra
AU  - Teixeira RA
AD  - Post-Graduation in Structural and Functional Biology, Federal University of Sao 
      Paulo (UNIFESP), Sao Paulo, Brazil.
FAU - Mimura, Kallyne Kioko Oliveira
AU  - Mimura KK
AD  - Post-Graduation in Structural and Functional Biology, Federal University of Sao 
      Paulo (UNIFESP), Sao Paulo, Brazil.
FAU - Araujo, Leandro Pires
AU  - Araujo LP
AD  - Post-Graduation in Structural and Functional Biology, Federal University of Sao 
      Paulo (UNIFESP), Sao Paulo, Brazil.
FAU - Greco, Karin Vicente
AU  - Greco KV
AD  - Department of Surgical Research, Northwick Park Institute for Medical Research - 
      University College London, London, UK.
FAU - Oliani, Sonia Maria
AU  - Oliani SM
AD  - Post-Graduation in Structural and Functional Biology, Federal University of Sao 
      Paulo (UNIFESP), Sao Paulo, Brazil.
AD  - Department of Biology, Instituto de Biociencias, Letras e Ciencias Exatas 
      (IBILCE), Sao Paulo State University (UNESP), Sao Jose do Rio Preto, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130730
PL  - England
TA  - J Tissue Eng Regen Med
JT  - Journal of tissue engineering and regenerative medicine
JID - 101308490
RN  - 0 (Annexin A1)
RN  - 0 (Annexin A5)
RN  - 0 (Peptides)
RN  - 0 (Protective Agents)
RN  - 0 (annexin A1 peptide (2-26))
RN  - 83HN0GTJ6D (Cyclosporine)
RN  - AYI8EX34EU (Creatinine)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Allografts/*drug effects
MH  - Animals
MH  - Annexin A1/*pharmacology
MH  - Annexin A5/metabolism
MH  - Apoptosis/drug effects
MH  - Cell Movement/drug effects
MH  - Creatinine/urine
MH  - Cyclosporine/pharmacology
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Graft Survival/*drug effects
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Neutrophils/cytology/drug effects
MH  - Peptides/*pharmacology
MH  - Phosphorylation/drug effects
MH  - Protective Agents/pharmacology
MH  - *Skin Transplantation
OTO - NOTNLM
OT  - annexin A1
OT  - cyclosporine A
OT  - neutrophils
OT  - skin
OT  - transplantation
EDAT- 2013/07/31 06:00
MHDA- 2016/11/01 06:00
CRDT- 2013/07/31 06:00
PHST- 2012/10/04 00:00 [received]
PHST- 2013/04/15 00:00 [revised]
PHST- 2012/04/16 00:00 [accepted]
PHST- 2013/07/31 06:00 [entrez]
PHST- 2013/07/31 06:00 [pubmed]
PHST- 2016/11/01 06:00 [medline]
AID - 10.1002/term.1773 [doi]
PST - ppublish
SO  - J Tissue Eng Regen Med. 2016 Feb;10(2):E44-53. doi: 10.1002/term.1773. Epub 2013 
      Jul 30.