PMID- 23898641
OWN - NLM
STAT- MEDLINE
DCOM- 20150708
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 31
IP  - 4
DP  - 2013 Apr
TI  - [Monolithic molecularly imprinted column-high performance liquid chromatography 
      for enrichment and determination of trace cytokinins in plant samples].
PG  - 392-6
AB  - A method based on monolithic molecularly imprinted polymer enrichment combining 
      with high performance liquid chromatography (mMIP-HPLC) detection was developed 
      for the selective determination of trace cytokinins (CTKs) in plant samples. 
      Monolithic molecularly imprinted polymer (mMIP) column was prepared in stainless 
      steel tube by using kinetin as the template, methacrylic acid (MAA) as the 
      functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linker, 
      and toluene-dodecanol as the porogenic solvents. Compared with non-imprinted 
      polymer (NIP) monolith, the prepared mMIP exhibited selective separation ability, 
      good reproducibility and reusability, and high extraction efficiency in the 
      separation and enrichment of the four CTKs. Under the optimized experimental 
      conditions, mean recoveries were 91.9%, 80.0%, 87.5% and 50.2% for kinetin (K), 
      kinetin glucoside (KR) , trans-zeatin (tZ) and meta-topolin (mT), respectively, 
      with the corresponding RSDs less than 11.8%. The proposed mMIP-HPLC method was 
      successfully applied in the separation and determination of the four cytokinins 
      in different plant samples
FAU - Sun, Lin
AU  - Sun L
AD  - College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 
      541004, China.
FAU - Du, Fuyou
AU  - Du F
FAU - Ruan, Guihua
AU  - Ruan G
FAU - Huang, Yijia
AU  - Huang Y
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Cytokinins)
RN  - 0 (Methacrylates)
RN  - 0 (Polymers)
RN  - 0 (Solvents)
RN  - 1CS02G8656 (methacrylic acid)
RN  - 7BK5G69305 (ethylene dimethacrylate)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Cytokinins/*analysis
MH  - Methacrylates
MH  - *Molecular Imprinting
MH  - Plants/*chemistry
MH  - Polymers
MH  - Reproducibility of Results
MH  - Solid Phase Extraction
MH  - Solvents
EDAT- 2013/08/01 06:00
MHDA- 2015/07/15 06:00
CRDT- 2013/08/01 06:00
PHST- 2013/08/01 06:00 [entrez]
PHST- 2013/08/01 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
AID - 10.3724/sp.j.1123.2012.10036 [doi]
PST - ppublish
SO  - Se Pu. 2013 Apr;31(4):392-6. doi: 10.3724/sp.j.1123.2012.10036.