PMID- 23898950
OWN - NLM
STAT- MEDLINE
DCOM- 20140704
LR  - 20131125
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 61
IP  - 35
DP  - 2013 Sep 4
TI  - Metabolic phenotypes associated with high-temperature tolerance of Porphyra 
      haitanensis strains.
PG  - 8356-63
LID - 10.1021/jf402749a [doi]
AB  - Colored mutants of Porphyra haitanensis have superior production and quality 
      characteristics, with two mutants, Shengfu 1 (SF-1) and Shengfu 2 (SF-2), having 
      good high-temperature tolerances. To understand the molecular aspects of 
      high-temperature tolerance, this study comprehensively investigated the metabolic 
      differences between the high-temperature tolerant strains and wild type. Nuclear 
      magnetic resonance (NMR) methods identified 35 algal metabolites, including 
      sugars, amino acids, carboxylic acids, aldehydes, amines, and nucleotides. The 
      results indicated that the high-temperature tolerant strains had significantly 
      different metabolic phenotypes from the wild type. The high-temperature tolerant 
      mutants had significantly higher levels in a set of osmolytes consisting of 
      betaine, taurine, laminitol, and isofloridoside than the wild type, indicating 
      the particular importance of efficient osmoregulation for high-temperature 
      resistance. These findings provided essential metabolic information about 
      high-temperature adaptation for P. haitanensis and demonstrated NMR-based 
      metabolomics as a useful tool for understanding the metabolic features related to 
      resistance to stressors.
FAU - Ye, Yangfang
AU  - Ye Y
AD  - CAS Key Laboratory of Magnetic Resonance in Biological Systems, Centre for 
      Biospectroscopy and Metabonomics, State Key Laboratory of Magnetic Resonance and 
      Atomic and Molecular Physics, Wuhan Centre for Magnetic Resonance, Wuhan 
      Institute of Physics and Mathematics, Chinese Academy of Sciences , Wuhan 430071, 
      People's Republic of China.
FAU - Zhang, Limin
AU  - Zhang L
FAU - Yang, Rui
AU  - Yang R
FAU - Luo, Qijun
AU  - Luo Q
FAU - Chen, Haimin
AU  - Chen H
FAU - Yan, Xiaojun
AU  - Yan X
FAU - Tang, Huiru
AU  - Tang H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130823
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Galactosides)
RN  - 1EQV5MLY3D (Taurine)
RN  - 3SCV180C9W (Betaine)
RN  - 4649-46-1 (isofloridoside)
RN  - 472-95-7 (laminitol)
RN  - 4L6452S749 (Inositol)
SB  - IM
MH  - Betaine
MH  - Galactosides/analysis
MH  - *Hot Temperature
MH  - Inositol/analogs & derivatives/analysis
MH  - Magnetic Resonance Spectroscopy
MH  - *Metabolome
MH  - Mutation
MH  - Osmoregulation
MH  - Phenotype
MH  - Porphyra/classification/*genetics/*metabolism
MH  - Taurine
EDAT- 2013/08/01 06:00
MHDA- 2014/07/06 06:00
CRDT- 2013/08/01 06:00
PHST- 2013/08/01 06:00 [entrez]
PHST- 2013/08/01 06:00 [pubmed]
PHST- 2014/07/06 06:00 [medline]
AID - 10.1021/jf402749a [doi]
PST - ppublish
SO  - J Agric Food Chem. 2013 Sep 4;61(35):8356-63. doi: 10.1021/jf402749a. Epub 2013 
      Aug 23.