PMID- 23899153
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130801
LR  - 20140108
IS  - 1354-750X (Print)
IS  - 1354-750X (Linking)
VI  - 2
IP  - 1
DP  - 1997
TI  - Time- and dose-dependent biom arker responses in flounder (Platichthys flesus L.) 
      exposed to benzo[a]pyrene, 2,3,3',4,4', 5-hexachlorobiphenyl (PCB-156) and 
      cadmium.
PG  - 35-44
LID - 10.1080/135475097231959 [doi]
AB  - Responses in flounder (Platichthys flesus) towards benzo [a]pyrene (BaP), 
      2,3,3',4,4',5-hexachlorobiphenyl (PCB-156), and cadmium (Cd) were investigated in 
      time-course and dose-response studies of selected biomarkers. Measurements of 
      biliary fluorescent BaP metabolites and hepatic concentrations of PCB-156 and 
      cadmium showed that the injected toxicants were rapidly m obilized from the 
      muscle to the liver, but a depot effect was indicated in the highest dose groups 
      of BaP and PCB-156 (12 mg kg(-1) bodyweight). Clearest biomarker responses were 
      found in the induction of hepatic cytochrome P450 1A (CYP1A) enzymes as a 
      response towards BaP and PCB-156 exposure. Maximum induction of CYP1A dependent 
      7-ethoxyresorufin O-deethylase (EROD) activity was observed after 2 and 8 days in 
      BaP and PCB-156-treated flounder, respectively. Positive dose-effect 
      relationships were observed towards both compounds, but the CYP1A induction was 
      more persistent with PCB exposure than with BaP exposure. In Cd-exposed fish, the 
      hepatic level of metallothionein responded more slowly with highest levels 
      observed after 16 days in the time-study. In the combined BaP + Cd treatment, the 
      CYP1A induction was only slightly suppressed. Aspartate aminotransferase in serum 
      appeared to be responsive towards BaP, but also towards the acetone vehicle in 
      controls in the first part of the exposure period. Hematocrit as well as hepatic 
      activities of aldrin epoxidase, glutathione S-transferase, and UDP-glucuronyl 
      transferase were not responsive to any treatm ent in the present study. In 
      general, the results demonstrate that selected biom arkers in flounder are 
      responsive to PAH, PCB, and heavy metal pollutant exposure, indicating the 
      applicability of this species in future environmental pollution monitoring 
      programmes.
FAU - Beyer, J
AU  - Beyer J
FAU - Sandvik, M
AU  - Sandvik M
FAU - Skare, J U
AU  - Skare JU
FAU - Egaas, E
AU  - Egaas E
FAU - Hylland, K
AU  - Hylland K
FAU - Waagbo, R
AU  - Waagbo R
FAU - Goksoyr, A
AU  - Goksoyr A
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biomarkers
JT  - Biomarkers : biochemical indicators of exposure, response, and susceptibility to 
      chemicals
JID - 9606000
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 2013/08/01 06:00
PHST- 2013/08/01 06:00 [entrez]
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
AID - 10.1080/135475097231959 [doi]
PST - ppublish
SO  - Biomarkers. 1997;2(1):35-44. doi: 10.1080/135475097231959.