PMID- 23903688
OWN - NLM
STAT- MEDLINE
DCOM- 20141003
LR  - 20211021
IS  - 1414-431X (Electronic)
IS  - 0100-879X (Print)
IS  - 0100-879X (Linking)
VI  - 46
IP  - 8
DP  - 2013 Aug
TI  - In vitro proliferation and differentiation of hepatic oval cells and their 
      potential capacity for intrahepatic transplantation.
PG  - 681-8
LID - S0100-879X2013005032620 [pii]
LID - 10.1590/1414-431X20132620 [doi]
AB  - Hepatic oval cells (HOCs) are recognized as facultative liver progenitor cells 
      that play a role in liver regeneration after acute liver injury. Here, we 
      investigated the in vitro proliferation and differentiation characteristics of 
      HOCs in order to explore their potential capacity for intrahepatic 
      transplantation. Clusters or scattered HOCs were detected in the portal area and 
      interlobular bile duct in the liver of rats subjected to the modified 
      2-acetylaminofluorene and partial hepatectomy method. Isolated HOCs were positive 
      for c-kit and CD90 staining (99.8% and 88.8%, respectively), and negative for 
      CD34 staining (3.6%) as shown by immunostaining and flow cytometric analysis. In 
      addition, HOCs could be differentiated into hepatocytes and bile duct epithelial 
      cells after leukemia inhibitory factor deprivation. A two-cuff technique was used 
      for orthotopic liver transplantation, and HOCs were subsequently transplanted 
      into recipients. Biochemical indicators of liver function were assessed 4 weeks 
      after transplantation. HOC transplantation significantly prolonged the median 
      survival time and improved the liver function of rats receiving HOCs compared to 
      controls (P = 0.003, Student t-test). Administration of HOCs to rats also 
      receiving liver transplantation significantly reduced acute allograft rejection 
      compared to control liver transplant rats 3 weeks following transplantation 
      (rejection activity index score: control = 6.3 +/- 0.9; HOC = 3.5 +/- 1.5; P = 
      0.005). These results indicate that HOCs may be useful in therapeutic liver 
      regeneration after orthotopic liver transplantation.
FAU - Li, Z
AU  - Li Z
AD  - Liaocheng People's Hospital, Department of Hepatobiliary Surgery, 
      LiaochengShandong, China.
FAU - Chen, J
AU  - Chen J
FAU - Li, L
AU  - Li L
FAU - Ran, J H
AU  - Ran JH
FAU - Liu, J
AU  - Liu J
FAU - Gao, T X
AU  - Gao TX
FAU - Guo, B Y
AU  - Guo BY
FAU - Li, X H
AU  - Li XH
FAU - Liu, Z H
AU  - Liu ZH
FAU - Liu, G J
AU  - Liu GJ
FAU - Gao, Y C
AU  - Gao YC
FAU - Zhang, X L
AU  - Zhang XL
LA  - eng
PT  - Journal Article
DEP - 20130730
PL  - Brazil
TA  - Braz J Med Biol Res
JT  - Brazilian journal of medical and biological research = Revista brasileira de 
      pesquisas medicas e biologicas
JID - 8112917
SB  - IM
MH  - Animals
MH  - Cell Differentiation/*physiology
MH  - *Cell Proliferation
MH  - Cell Transplantation/*methods
MH  - Female
MH  - Flow Cytometry
MH  - Graft Rejection/diagnosis
MH  - Hepatectomy
MH  - Hepatocytes/*cytology
MH  - Immunohistochemistry
MH  - Liver/anatomy & histology/surgery
MH  - Liver Transplantation/*methods
MH  - Male
MH  - Primary Cell Culture
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Real-Time Polymerase Chain Reaction/methods
MH  - Survival Rate
PMC - PMC3854420
EDAT- 2013/08/02 06:00
MHDA- 2014/10/04 06:00
PMCR- 2013/07/30
CRDT- 2013/08/02 06:00
PHST- 2012/11/07 00:00 [received]
PHST- 2013/03/25 00:00 [accepted]
PHST- 2013/08/02 06:00 [entrez]
PHST- 2013/08/02 06:00 [pubmed]
PHST- 2014/10/04 06:00 [medline]
PHST- 2013/07/30 00:00 [pmc-release]
AID - S0100-879X2013005032620 [pii]
AID - 10.1590/1414-431X20132620 [doi]
PST - ppublish
SO  - Braz J Med Biol Res. 2013 Aug;46(8):681-8. doi: 10.1590/1414-431X20132620. Epub 
      2013 Jul 30.