PMID- 23903843
OWN - NLM
STAT- MEDLINE
DCOM- 20131227
LR  - 20230620
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 87
IP  - 19
DP  - 2013 Oct
TI  - Herpes simplex virus 1 targets the murine olfactory neuroepithelium for host 
      entry.
PG  - 10477-88
LID - 10.1128/JVI.01748-13 [doi]
AB  - Herpes simplex virus 1 (HSV-1) is a ubiquitous and important human pathogen. It 
      is known to persist in trigeminal ganglia (TG), but how it reaches this site has 
      been difficult to determine, as viral transmission is sporadic, pathogenesis is 
      complicated, and early infection is largely asymptomatic. We used mice to compare 
      the most likely natural HSV-1 host entry routes: oral and nasal. Intranasal 
      infection was 100-fold more efficient than oral and targeted predominantly the 
      olfactory neuroepithelium. Live imaging of HSV-1-expressed luciferase showed 
      infection progressing from the nose to the TG and then reemerging in the facial 
      skin. The brain remained largely luciferase negative throughout. Infected cell 
      tagging by viral Cre recombinase expression in floxed reporter gene mice showed 
      nasal virus routinely reaching the TG and only rarely reaching the olfactory 
      bulbs. Thus, HSV-1 spread from the olfactory neuroepithelium to the TG and 
      reemerged peripherally without causing significant neurological disease. This 
      recapitulation of typical clinical infection suggests that HSV-1 might sometimes 
      also enter humans via the respiratory tract.
FAU - Shivkumar, Maitreyi
AU  - Shivkumar M
AD  - Division of Virology, Department of Pathology, University of Cambridge, 
      Addenbrookes Hospital, Cambridge, United Kingdom.
FAU - Milho, Ricardo
AU  - Milho R
FAU - May, Janet S
AU  - May JS
FAU - Nicoll, Michael P
AU  - Nicoll MP
FAU - Efstathiou, Stacey
AU  - Efstathiou S
FAU - Stevenson, Philip G
AU  - Stevenson PG
LA  - eng
GR  - WT089111MA/WT_/Wellcome Trust/United Kingdom
GR  - MR/J002232/1/MRC_/Medical Research Council/United Kingdom
GR  - J014419/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - BB/J014419/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - G0701185/MRC_/Medical Research Council/United Kingdom
GR  - WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130731
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fluorescent Antibody Technique
MH  - Herpes Simplex/genetics/pathology/*virology
MH  - Herpesvirus 1, Human/*pathogenicity
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Kidney/metabolism/pathology/virology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Neuroepithelial Cells/metabolism/pathology/*virology
MH  - Olfactory Bulb/metabolism/pathology/*virology
MH  - Trigeminal Ganglion/metabolism/pathology/*virology
MH  - *Virus Internalization
MH  - Virus Replication
PMC - PMC3807398
EDAT- 2013/08/02 06:00
MHDA- 2013/12/29 06:00
PMCR- 2013/10/01
CRDT- 2013/08/02 06:00
PHST- 2013/08/02 06:00 [entrez]
PHST- 2013/08/02 06:00 [pubmed]
PHST- 2013/12/29 06:00 [medline]
PHST- 2013/10/01 00:00 [pmc-release]
AID - JVI.01748-13 [pii]
AID - 01748-13 [pii]
AID - 10.1128/JVI.01748-13 [doi]
PST - ppublish
SO  - J Virol. 2013 Oct;87(19):10477-88. doi: 10.1128/JVI.01748-13. Epub 2013 Jul 31.