PMID- 23904044
OWN - NLM
STAT- MEDLINE
DCOM- 20140317
LR  - 20220309
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 8
IP  - 4
DP  - 2013 Oct
TI  - Lycium barbarum polysaccharides induce Toll-like receptor 2- and 4-mediated 
      phenotypic and functional maturation of murine dendritic cells via activation of 
      NF-kappaB.
PG  - 1216-20
LID - 10.3892/mmr.2013.1608 [doi]
AB  - Dendritic cells (DCs) are potent antigen‑presenting cells that play pivotal roles 
      in the initiation of primary immune responses. Lycium barbarum polysaccharides 
      (LBPs) are known to have a variety of immunomodulatory functions. However, the 
      cellular and molecular mechanisms underlying their therapeutic effects are poorly 
      understood. In this study, we report that LBPs induce phenotypic and functional 
      maturation of DCs. LBPs upregulated DC expression of I‑A/I‑E and CD11c, enhanced 
      DC allostimulatory activity and induced IL‑12p40 production. Furthermore, the 
      activity of LBPs on DCs was significantly reduced by treating the cells with 
      anti‑TLR2 or anti‑TLR4 antibody prior to LBPs, indicating that both are possible 
      receptors of LBPs. Maturation of DCs by LBPs was able to directly activate the 
      nuclear transcription factor NF‑kappaB p65. The results revealed that LBP stimulation 
      induces the phenotypic and functional maturation of DCs via TLR2- and/or 
      TLR4-mediated NF‑kappaB signaling pathways.
FAU - Zhu, Jie
AU  - Zhu J
AD  - Department of Clinical Laboratory, Zhejiang Hospital, Hangzhou, Zhejiang 310013, 
      P.R. China. dawnnyzj@yahoo.com.cn
FAU - Zhang, Yuanyuan
AU  - Zhang Y
FAU - Shen, Yueshuang
AU  - Shen Y
FAU - Zhou, Huanqin
AU  - Zhou H
FAU - Yu, Xiaomei
AU  - Yu X
LA  - eng
PT  - Journal Article
DEP - 20130731
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (CD11c Antigen)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Interleukin-12 Subunit p40)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Tlr2 protein, mouse)
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (lycium barbarum polysaccharide)
SB  - IM
MH  - Animals
MH  - CD11c Antigen/metabolism
MH  - Cell Proliferation
MH  - Coculture Techniques
MH  - Dendritic Cells/drug effects/immunology/*metabolism
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Female
MH  - Interleukin-12 Subunit p40/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Lycium/chemistry
MH  - Lymphocyte Culture Test, Mixed
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - NF-kappa B/metabolism
MH  - Phenotype
MH  - Signal Transduction
MH  - Toll-Like Receptor 2/*metabolism
MH  - Toll-Like Receptor 4/*metabolism
EDAT- 2013/08/02 06:00
MHDA- 2014/03/19 06:00
CRDT- 2013/08/02 06:00
PHST- 2013/03/12 00:00 [received]
PHST- 2013/07/22 00:00 [accepted]
PHST- 2013/08/02 06:00 [entrez]
PHST- 2013/08/02 06:00 [pubmed]
PHST- 2014/03/19 06:00 [medline]
AID - 10.3892/mmr.2013.1608 [doi]
PST - ppublish
SO  - Mol Med Rep. 2013 Oct;8(4):1216-20. doi: 10.3892/mmr.2013.1608. Epub 2013 Jul 31.