PMID- 23904221
OWN - NLM
STAT- MEDLINE
DCOM- 20140210
LR  - 20191224
IS  - 1522-1466 (Electronic)
IS  - 1522-1466 (Linking)
VI  - 305
IP  - 11
DP  - 2013 Dec 1
TI  - Anti-C5a complementary peptide ameliorates acute peritoneal injury induced by 
      neutralization of Crry and CD59.
PG  - F1603-16
LID - 10.1152/ajprenal.00681.2012 [doi]
AB  - In peritoneal dialysis (PD) therapy, physical stresses such as exposure to 
      peritoneal dialysate, catheter trauma, and peritonitis may induce peritoneal 
      injury that can prevent continued long-term PD therapy. Therefore, protection of 
      the peritoneum is an important target to enable long-term PD therapy in patients 
      with end-stage renal disease. We previously showed that neutralization of the 
      membrane complement regulators (CRegs) Crry and CD59 in rat peritoneum provokes 
      development of acute peritoneal injury due to uncontrolled complement activation. 
      C5a is a key effecter molecule of the complement system released during acute 
      inflammation. Control of C5a has been proposed as a strategy to suppress 
      inflammatory reactions and, because peritoneal injury is accompanied by 
      inflammation, we hypothesized that C5a targeted therapy might be an effective way 
      to suppress peritoneal injury. In the present study we used an established acute 
      peritonitis model induced by neutralization of CRegs to investigate the effects 
      on acute peritoneal injury of inhibiting C5a. Intravenous administration of an 
      anti-C5a complementary peptide (AcPepA) up to 4 h after induction of injury 
      significantly and dose-dependently prevented accumulation of inflammatory cells 
      and reduced tissue damage in the model, accompanied by decreased C3b deposition. 
      We show that C5a contributed to the development of peritoneal injury. Our results 
      suggest that C5a is a target for preventing or treating peritoneal injury in 
      patients undergoing prolonged PD therapy or with infectious complications.
FAU - Mizuno, Tomohiro
AU  - Mizuno T
AD  - Renal Replacement Therapy, Division of Nephrology, Nagoya Univ. Graduate School 
      of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan. mmizu@med.nagoya-u.ac.jp 
      or masashim1jp@yahoo.co.jp.
FAU - Mizuno, Masashi
AU  - Mizuno M
FAU - Imai, Masaki
AU  - Imai M
FAU - Suzuki, Yasuhiro
AU  - Suzuki Y
FAU - Kushida, Mayu
AU  - Kushida M
FAU - Noda, Yukihiro
AU  - Noda Y
FAU - Maruyama, Shoichi
AU  - Maruyama S
FAU - Okada, Hidechika
AU  - Okada H
FAU - Okada, Noriko
AU  - Okada N
FAU - Matsuo, Seiichi
AU  - Matsuo S
FAU - Ito, Yasuhiko
AU  - Ito Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130731
PL  - United States
TA  - Am J Physiol Renal Physiol
JT  - American journal of physiology. Renal physiology
JID - 100901990
RN  - 0 (Antibodies)
RN  - 0 (Antigens, Protozoan)
RN  - 0 (Antigens, Surface)
RN  - 0 (C5 phagosomal antigen, Paramecium multimicronucleatum)
RN  - 0 (CD59 Antigens)
RN  - 0 (Cr1l protein, rat)
RN  - 0 (Peptide Fragments)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Animals
MH  - Antibodies/immunology/*therapeutic use
MH  - Antigens, Protozoan/*immunology
MH  - Antigens, Surface/*immunology/*metabolism
MH  - CD59 Antigens/*metabolism
MH  - Complement Activation/drug effects/immunology
MH  - Disease Models, Animal
MH  - Male
MH  - Peptide Fragments/immunology/pharmacology
MH  - Peritoneal Dialysis/methods
MH  - Peritoneum/*drug effects/immunology/injuries
MH  - Peritonitis/chemically induced/*drug therapy/immunology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Cell Surface/*metabolism
OTO - NOTNLM
OT  - C5a
OT  - complement
OT  - peritoneal dialysis
OT  - peritoneal injury
OT  - peritonitis
EDAT- 2013/08/02 06:00
MHDA- 2014/02/11 06:00
CRDT- 2013/08/02 06:00
PHST- 2013/08/02 06:00 [entrez]
PHST- 2013/08/02 06:00 [pubmed]
PHST- 2014/02/11 06:00 [medline]
AID - ajprenal.00681.2012 [pii]
AID - 10.1152/ajprenal.00681.2012 [doi]
PST - ppublish
SO  - Am J Physiol Renal Physiol. 2013 Dec 1;305(11):F1603-16. doi: 
      10.1152/ajprenal.00681.2012. Epub 2013 Jul 31.