PMID- 23904365
OWN - NLM
STAT- MEDLINE
DCOM- 20140909
LR  - 20211021
IS  - 1672-0733 (Print)
IS  - 1672-0733 (Linking)
VI  - 33
IP  - 4
DP  - 2013 Aug
TI  - Role of brain-derived neurotrophic factor in bone marrow angiogenesis in multiple 
      myeloma.
PG  - 485-490
LID - 10.1007/s11596-013-1146-3 [doi]
AB  - This study examined the expression of brain-derived neurotrophic factor (BDNF) in 
      multiple myeloma (MM) and its role in bone marrow angiogenesis. The peripheral 
      blood plasma was harvested from 71 MM patients and 63 patients without 
      hematological malignancy. The BDNF level in the blood plasma was determined by 
      ELISA. Human bone marrow endothelial cells (HBMECs) were cultured. The mRNA and 
      protein expression levels of the BDNF receptor TrkB in HBMECs were detected by 
      using RT-PCR and flow cytometry, respectively. The viability of HBMECs treated 
      with recombinant human (rh) BDNF or not was measured by using MTT assay. The 
      migration of HBMECs in the presence of rhBDNF or not was determined by modified 
      Boyden chamber assay. In vitro tube formation assay was used to assess the effect 
      of rhBDNF on HBMECs differentiation. The results of ELISA revealed that the BDNF 
      level was significantly higher in peripheral blood plasma of MM patients than in 
      that of control patients (4.39+/-0.67 vs. 1.96+/-0.39 ng/mL, P<0.05). The BDNF 
      receptor TrkB was expressed in HBMECs at mRNA and protein level. MTT assay 
      manifested that rhBDNF could significantly concentration-dependently promote the 
      HBMECs proliferation. The number of HBMECs treated with 160 ng/mL rhBDNF for 48 h 
      was 1.57+/-0.10 folds higher than that in control group (P<0.05). Moreover, rhBDNF 
      could enhance HBMECs migration in a concentration-dependent manner and the 
      maximal migration was reached in the presence of 100 ng/mL rhBDNF. The migration 
      indexes were 1.40+/-0.11, 1.64+/-0.16, 2.06+/-0.25 and 2.18+/-0.21 in 25, 50, 100 ng/mL 
      rhBDNF groups and 25 ng/mL rhVEGF group, respectively. In vitro tube formation 
      assay demonstrated that the area of the formed tubular structure was increased 
      with the rhBDNF concentration. In control group, there was no formation of intact 
      tubular structure and the HBMECs on the matrigel were irregularly dispersed. 
      HBMECs treated with 100 ng/mL rhBDNF could form intact tubular structure and the 
      area and the diameter of tubes were significantly greater than those in control 
      group (P<0.05). There was no significant difference in the formed tubular area 
      between 25 ng/mL VEGF group and 100 ng/mL rhBDNF group. It was concluded that 
      BDNF plays an important role in myeloma cell-induced angiogenesis, and it may 
      become a new target of anti-angiogenesis treatment for MM.
FAU - Chu, Zhang-Bo
AU  - Chu ZB
AD  - Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430022, China.
FAU - Sun, Chun-Yan
AU  - Sun CY
AD  - Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430022, China.
FAU - Yang, Di
AU  - Yang D
AD  - Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430022, China.
FAU - Chen, Lei
AU  - Chen L
AD  - Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430022, China.
FAU - Hu, Yu
AU  - Hu Y
AD  - Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430022, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130801
PL  - China
TA  - J Huazhong Univ Sci Technolog Med Sci
JT  - Journal of Huazhong University of Science and Technology. Medical sciences = Hua 
      zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. 
      Yixue Yingdewen ban
JID - 101169627
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 7171WSG8A2 (BDNF protein, human)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Bone Marrow/*metabolism/pathology
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/*metabolism/pathology
MH  - Neovascularization, Pathologic/*metabolism/pathology
EDAT- 2013/08/02 06:00
MHDA- 2014/09/10 06:00
CRDT- 2013/08/02 06:00
PHST- 2012/12/26 00:00 [received]
PHST- 2013/03/26 00:00 [revised]
PHST- 2013/08/02 06:00 [entrez]
PHST- 2013/08/02 06:00 [pubmed]
PHST- 2014/09/10 06:00 [medline]
AID - 10.1007/s11596-013-1146-3 [pii]
AID - 10.1007/s11596-013-1146-3 [doi]
PST - ppublish
SO  - J Huazhong Univ Sci Technolog Med Sci. 2013 Aug;33(4):485-490. doi: 
      10.1007/s11596-013-1146-3. Epub 2013 Aug 1.