PMID- 23906910
OWN - NLM
STAT- MEDLINE
DCOM- 20140217
LR  - 20130802
IS  - 1559-6095 (Electronic)
IS  - 1559-6095 (Linking)
VI  - 2013
IP  - 8
DP  - 2013 Aug 1
TI  - Live cell imaging of cytoplasmic and nuclear Ca2+ dynamics in Arabidopsis roots.
PG  - 776-80
LID - 10.1101/pdb.prot073031 [doi]
AB  - Approaches for Ca(2+) measurement require data recording with high spatiotemporal 
      resolution. This is achieved by combined usage of locally targeted genetically 
      encoded calcium indicators (GECIs) and confocal laser scanning microscopy (CLSM). 
      This protocol provides instructions for setting up a CLSM-based experiment to 
      measure cytosolic and nuclear Ca(2+) dynamics in Arabidopsis lines expressing 
      Yellow Cameleon Ca(2+) indicators.
FAU - Krebs, Melanie
AU  - Krebs M
AD  - Department of Developmental Biology, Centre for Organismal Studies, University of 
      Heidelberg, 69120 Heidelberg, Germany. melanie.krebs@cos.uni-heidelberg.de
FAU - Schumacher, Karin
AU  - Schumacher K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130801
PL  - United States
TA  - Cold Spring Harb Protoc
JT  - Cold Spring Harbor protocols
JID - 101524530
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Cations, Divalent)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (yellow cameleon)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Arabidopsis/cytology
MH  - Calcium/*analysis
MH  - Calcium-Binding Proteins/genetics/*metabolism
MH  - Cations, Divalent/analysis
MH  - Cell Nucleus/*chemistry
MH  - Cytological Techniques/*methods
MH  - Cytoplasm/*chemistry
MH  - Fluorescent Dyes/*metabolism
MH  - Microscopy, Confocal/*methods
MH  - Plant Roots/cytology
MH  - Staining and Labeling/methods
MH  - Transgenes
EDAT- 2013/08/03 06:00
MHDA- 2014/02/18 06:00
CRDT- 2013/08/03 06:00
PHST- 2013/08/03 06:00 [entrez]
PHST- 2013/08/03 06:00 [pubmed]
PHST- 2014/02/18 06:00 [medline]
AID - 2013/8/pdb.prot073031 [pii]
AID - 10.1101/pdb.prot073031 [doi]
PST - epublish
SO  - Cold Spring Harb Protoc. 2013 Aug 1;2013(8):776-80. doi: 10.1101/pdb.prot073031.