PMID- 23907946
OWN - NLM
STAT- MEDLINE
DCOM- 20140221
LR  - 20161020
IS  - 1897-5631 (Electronic)
IS  - 0239-8508 (Linking)
VI  - 51
IP  - 2
DP  - 2013
TI  - Effect of unfractionated heparin, enoxaparin and sulodexide on the relations 
      between secretion and expression of OPG, RANKL and vWF in HUVEC.
PG  - 156-63
LID - 10.5603/FHC.2013.0016 [doi]
AB  - Heparin modulates function of vascular endothelium. We studied the effects of 
      unfractionated heparin (UFH) vs. enoxaparin vs. sulodexide on the levels and gene 
      expression of osteoprotegerin (OPG), Receptor Activator of Nuclear Factor kB 
      Ligand (RANKL) and von Willebrand factor (vWF) in Human Umbilical Vein 
      Endothelial Cells (HUVEC) culture. HUVEC were isolated from human umbilical vein 
      by a standard method. The supernatant concentrations (ELISA) and gene expression 
      (Real Time-PCR) of OPG, RANKL and vWF in HUVEC were determined after incubation 
      with various concentrations of UFH, enoxaparin and sulodexide for up to 16 hours. 
      In control HUVEC strong positive correlation between OPG and vWF levels was 
      observed, whereas sRANKL negatively correlated with OPG and vWF levels. Only in 
      control HUVEC a negative correlation between the supernatant level of vWF and its 
      gene expression was found. Already the lowest concentration of UFH caused 
      2.5-fold increase in OPG gene expression while higher UFH concentrations 
      substantially increased RANKL mRNA level. A negative correlation between the OPG 
      and sRANKL concentration was noticed in supernatant HUVEC which were incubated 
      with enoxaparine. In conclusion, the observed interrelationships between OPG, 
      RANKL and vWF levels in unstimulated HUVEC support the presumption of the 
      pathophysiological links between these proteins. Of the tested heparin formulas 
      UFH seems to be the most potent in altering the OPG, RANKL and vWF axis.
FAU - Lekesiz, Katarzyna
AU  - Lekesiz K
AD  - Department of Nephrology and Transplantation with Dialysis Unit, Medical 
      University, Bialystok, Poland.
FAU - Naumnik, Beata
AU  - Naumnik B
FAU - Borysewicz-Sanczyk, Hanna
AU  - Borysewicz-Sanczyk H
FAU - Koc-Zurawska, Ewa
AU  - Koc-Zurawska E
FAU - Mysliwiec, Michal
AU  - Mysliwiec M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Poland
TA  - Folia Histochem Cytobiol
JT  - Folia histochemica et cytobiologica
JID - 8502651
RN  - 0 (Anticoagulants)
RN  - 0 (Enoxaparin)
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Osteoprotegerin)
RN  - 0 (RANK Ligand)
RN  - 0 (von Willebrand Factor)
RN  - 75HGV0062C (glucuronyl glucosamine glycan sulfate)
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Anticoagulants/*pharmacology
MH  - Enoxaparin/pharmacology
MH  - Exocytosis
MH  - Glycosaminoglycans/*pharmacology
MH  - Heparin/*pharmacology
MH  - Human Umbilical Vein Endothelial Cells/drug effects/*metabolism
MH  - Humans
MH  - Osteoprotegerin/genetics/*metabolism
MH  - RANK Ligand/genetics/*metabolism
MH  - Transcription, Genetic
MH  - von Willebrand Factor/genetics/*metabolism
EDAT- 2013/08/03 06:00
MHDA- 2014/02/22 06:00
CRDT- 2013/08/03 06:00
PHST- 2013/05/17 00:00 [received]
PHST- 2013/06/19 00:00 [accepted]
PHST- 2013/06/06 00:00 [revised]
PHST- 2013/08/03 06:00 [entrez]
PHST- 2013/08/03 06:00 [pubmed]
PHST- 2014/02/22 06:00 [medline]
AID - VM/OJS/J/34640 [pii]
AID - 10.5603/FHC.2013.0016 [doi]
PST - ppublish
SO  - Folia Histochem Cytobiol. 2013;51(2):156-63. doi: 10.5603/FHC.2013.0016.