PMID- 23908036
OWN - NLM
STAT- MEDLINE
DCOM- 20131226
LR  - 20240323
IS  - 1744-3091 (Electronic)
IS  - 1744-3091 (Linking)
VI  - 69
IP  - Pt 8
DP  - 2013 Aug
TI  - Preliminary X-ray crystallographic analysis of beta-carbonic anhydrase psCA3 from 
      Pseudomonas aeruginosa.
PG  - 891-4
LID - 10.1107/S1744309113017594 [doi]
AB  - Pseudomonas aeruginosa is a Gram-negative bacterium that causes life-threatening 
      infections in susceptible individuals and is resistant to most clinically 
      available antimicrobials. Genomic and proteomic studies have identified three 
      genes, pa0102, pa2053 and pa4676, in P. aeruginosa PAO1 encoding three functional 
      beta-carbonic anhydrases (beta-CAs): psCA1, psCA2 and psCA3, respectively. These beta-CAs 
      could serve as novel antimicrobial drug targets for this pathogen. X-ray 
      crystallographic structural studies have been initiated to characterize the 
      structure and function of these proteins. This communication describes the 
      production of two crystal forms (A and B) of beta-CA psCA3. Form A diffracted to a 
      resolution of 2.9 A; it belonged to space group P212121, with unit-cell 
      parameters a = 81.9, b = 84.9, c = 124.2 A, and had a calculated Matthews 
      coefficient of 2.23 A(3) Da(-)(1) assuming four molecules in the crystallographic 
      asymmetric unit. Form B diffracted to a resolution of 3.0 A; it belonged to space 
      group P21212, with unit-cell parameters a = 69.9, b = 77.7, c = 88.5 A, and had a 
      calculated Matthews coefficient of 2.48 A(3) Da(-)(1) assuming two molecules in the 
      crystallographic asymmetric unit. Preliminary molecular-replacement solutions 
      have been determined with the PHENIX AutoMR wizard and refinement of both crystal 
      forms is currently in progress.
FAU - Pinard, Melissa
AU  - Pinard M
AD  - Department of Biochemistry and Molecular Biology, University of Florida, PO Box 
      100245, Gainesville, FL 32610, USA.
FAU - Lotlikar, Shalaka
AU  - Lotlikar S
FAU - Patrauchan, Marianna A
AU  - Patrauchan MA
FAU - McKenna, Robert
AU  - McKenna R
LA  - eng
GR  - R01 GM025154/GM/NIGMS NIH HHS/United States
GR  - GM25154/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20130727
PL  - England
TA  - Acta Crystallogr Sect F Struct Biol Cryst Commun
JT  - Acta crystallographica. Section F, Structural biology and crystallization 
      communications
JID - 101226117
RN  - 0 (Bacterial Proteins)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
SB  - IM
MH  - Bacterial Proteins/biosynthesis/*chemistry
MH  - Carbonic Anhydrases/biosynthesis/*chemistry
MH  - Crystallography, X-Ray
MH  - Pseudomonas aeruginosa/*enzymology
PMC - PMC3729167
OTO - NOTNLM
OT  - Pseudomonas aeruginosa
OT  - carbonic anhydrases
OT  - beta-class carbonic anhydrases
EDAT- 2013/08/03 06:00
MHDA- 2013/12/27 06:00
PMCR- 2014/08/01
CRDT- 2013/08/03 06:00
PHST- 2013/05/17 00:00 [received]
PHST- 2013/06/26 00:00 [accepted]
PHST- 2013/08/03 06:00 [entrez]
PHST- 2013/08/03 06:00 [pubmed]
PHST- 2013/12/27 06:00 [medline]
PHST- 2014/08/01 00:00 [pmc-release]
AID - S1744309113017594 [pii]
AID - nj5159 [pii]
AID - 10.1107/S1744309113017594 [doi]
PST - ppublish
SO  - Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Aug;69(Pt 8):891-4. doi: 
      10.1107/S1744309113017594. Epub 2013 Jul 27.