PMID- 23908683
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130805
LR  - 20211021
IS  - 1936-5233 (Print)
IS  - 1936-5233 (Electronic)
IS  - 1936-5233 (Linking)
VI  - 6
IP  - 4
DP  - 2013 Aug
TI  - Comprehensive Analysis of ETS Family Members in Melanoma by Fluorescence In Situ 
      Hybridization Reveals Recurrent ETV1 Amplification.
PG  - 405-12
AB  - E26 transformation-specific (ETS) transcription factors are known to be involved 
      in gene aberrations in various malignancies including prostate cancer; however, 
      their role in melanoma oncogenesis has yet to be fully explored. We have 
      completed a comprehensive fluorescence in situ hybridization (FISH)-based screen 
      for all 27 members of the ETS transcription factor family on two melanoma tissue 
      microarrays, representing 223 melanomas, 10 nevi, and 5 normal skin tissues. None 
      of the melanoma cases demonstrated ETS fusions; however, 6 of 114 (5.3%) 
      melanomas were amplified for ETV1 using a break-apart FISH probe. For the six 
      positive cases, locus-controlled FISH probes revealed that two of six cases were 
      amplified for the ETV1 region, whereas four cases showed copy gains of the entire 
      chromosome 7. The remaining 26 ETS family members showed no chromosomal 
      aberrations by FISH. Quantitative polymerase chain reaction showed an average 
      3.4-fold (P value = .00218) increased expression of ETV1 in melanomas, including 
      the FISH ETV1-amplified cases, when compared to other malignancies (prostate, 
      breast, and bladder carcinomas). These data suggest that a subset of melanomas 
      overexpresses ETV1 and amplification of ETV1 may be one mechanism for achieving 
      high gene expression.
FAU - Mehra, Rohit
AU  - Mehra R
AD  - Michigan Center for Translational Pathology, University of Michigan Medical 
      School, Ann Arbor, MI ; Department of Pathology, University of Michigan Medical 
      School, Ann Arbor, MI ; The Comprehensive Cancer Center, University of Michigan 
      Medical School, Ann Arbor, MI.
FAU - Dhanasekaran, Saravana M
AU  - Dhanasekaran SM
FAU - Palanisamy, Nallasivam
AU  - Palanisamy N
FAU - Vats, Pankaj
AU  - Vats P
FAU - Cao, Xuhong
AU  - Cao X
FAU - Kim, Jung H
AU  - Kim JH
FAU - Kim, David Sl
AU  - Kim DS
FAU - Johnson, Timothy
AU  - Johnson T
FAU - Fullen, Douglas R
AU  - Fullen DR
FAU - Chinnaiyan, Arul M
AU  - Chinnaiyan AM
LA  - eng
PT  - Journal Article
DEP - 20130801
PL  - United States
TA  - Transl Oncol
JT  - Translational oncology
JID - 101472619
PMC - PMC3730015
EDAT- 2013/08/03 06:00
MHDA- 2013/08/03 06:01
PMCR- 2013/08/01
CRDT- 2013/08/03 06:00
PHST- 2013/04/15 00:00 [received]
PHST- 2013/05/02 00:00 [revised]
PHST- 2013/05/05 00:00 [accepted]
PHST- 2013/08/03 06:00 [entrez]
PHST- 2013/08/03 06:00 [pubmed]
PHST- 2013/08/03 06:01 [medline]
PHST- 2013/08/01 00:00 [pmc-release]
AID - 13340 [pii]
AID - 10.1593/tlo.13340 [doi]
PST - epublish
SO  - Transl Oncol. 2013 Aug 1;6(4):405-12. doi: 10.1593/tlo.13340. Print 2013 Aug.