PMID- 23910993
OWN - NLM
STAT- MEDLINE
DCOM- 20141209
LR  - 20140402
IS  - 1752-699X (Electronic)
IS  - 1752-6981 (Linking)
VI  - 8
IP  - 2
DP  - 2014 Apr
TI  - Antiphospholipid IgM antibody response in acute and chronic Mycobacterium 
      tuberculosis mouse infection model.
PG  - 137-44
LID - 10.1111/crj.12049 [doi]
AB  - BACKGROUND AND AIMS: The clinical management of tuberculosis (TB) could be 
      greatly improved by an affordable biomarker test to monitor treatment response. 
      Here, we examined changes in immunoglobulin M (IgM) antibody response to lipids 
      as a potential biomarker for monitoring TB treatment in an experimental mouse 
      model. METHODS: We performed enzyme-linked immunosorbent assay to investigate 
      changes in IgM antibody response against cardiolipin (CL), phosphatidylcholine 
      (PTC), phosphatidylethanolamine (PE), phosphatidylinositol (PI) and sphingolipid 
      (SL) in BALB/c mice that were treated after being infected with Mycobacterium 
      tuberculosis for 4 weeks (acute infection) and 20 weeks (chronic infection). 
      Cytokine levels [interleukin (IL)-5, IL-10, interferon-gamma (IFN-gamma), monocyte 
      chemoattractant protein-1 (MCP-1)] in lung and spleen homogenates as well as in 
      blood were also compared. RESULTS: In both acutely and chronically infected mice, 
      lungs were sterilised of M. tuberculosis infection after 8 weeks of treatment. 
      The IgM response to CL, PTC, PE, PI and SL were consistently elevated throughout 
      the course of infection in chronically infected mice compared with acutely 
      infected mice. In acutely infected mice, the IgM antibody response against CL 
      significantly decreased after 8 weeks of treatment, but not against other lipids. 
      In chronically infected mice, the IgM response showed no significant changes 
      against any of the lipids after 8 weeks of treatment. Of the cytokines examined, 
      only MCP-1 levels in lungs decreased significantly after treatment. CONCLUSION: 
      These findings demonstrate that antilipid IgM antibody can remain elevated in 
      chronically infected mice, but with treatment, only anti-CL IgM antibody levels 
      decreased together with M. tuberculosis bacterial burden in acutely infected 
      mice. Treatment did not affect antilipid IgM levels in chronically infected mice.
CI  - (c) 2013 John Wiley & Sons Ltd.
FAU - Goodridge, Amador
AU  - Goodridge A
AD  - Institute of Scientific Research and High Technology Services (INDICASAT-AIP), 
      City of Knowledge, Panama, Panama; Division of Infectious Diseases and 
      Vaccinology, School of Public Health, University of California, Berkeley, CA, 
      USA.
FAU - Zhang, Tianyi
AU  - Zhang T
FAU - Miyata, Toshiko
AU  - Miyata T
FAU - Lu, Sangwei
AU  - Lu S
FAU - Riley, Lee W
AU  - Riley LW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131001
PL  - England
TA  - Clin Respir J
JT  - The clinical respiratory journal
JID - 101315570
RN  - 0 (Antibodies, Antiphospholipid)
RN  - 0 (Antitubercular Agents)
RN  - 0 (Immunoglobulin M)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Antibodies, Antiphospholipid/*immunology
MH  - Antitubercular Agents/therapeutic use
MH  - Chronic Disease
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Follow-Up Studies
MH  - *Immunity, Innate
MH  - Immunoglobulin M/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Tuberculosis/drug therapy/*immunology/microbiology
OTO - NOTNLM
OT  - B-1 B cell
OT  - IgM
OT  - antiphospholipid
OT  - biomarker
OT  - monitoring
OT  - treatment
OT  - tuberculosis
EDAT- 2013/08/06 06:00
MHDA- 2014/12/15 06:00
CRDT- 2013/08/06 06:00
PHST- 2013/02/27 00:00 [received]
PHST- 2013/06/24 00:00 [revised]
PHST- 2013/07/25 00:00 [accepted]
PHST- 2013/08/06 06:00 [entrez]
PHST- 2013/08/06 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - 10.1111/crj.12049 [doi]
PST - ppublish
SO  - Clin Respir J. 2014 Apr;8(2):137-44. doi: 10.1111/crj.12049. Epub 2013 Oct 1.